Protein Variants | Comment | Organism |
---|---|---|
additional information | the cytotoxic enzyme induces apoptosis in transformed cells | Aralia elata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fucose | glucose, mannose, and N-acetylglucosamine show poor inhibitory effects | Aralia elata | |
galactose | and derivatives, inhibits the incorporation of the enzyme into target cells via its cell surface receptor, and inhibits the inhibition of protein synthesis through the enzyme, minimum inhibition concentration is 3.3 mM | Aralia elata | |
lactose | minimum inhibition concentration is 0.8 mM | Aralia elata | |
melibiose | minimum inhibition concentration is 1.0 mM | Aralia elata | |
N-acetylgalactosamine | minimum inhibition concentration is 4.2 mM | Aralia elata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme enters target cells via its cell surface receptor | Aralia elata | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
1 * 29000, A-chain, + 1 * 32000, B-chain, SDS-PAGE | Aralia elata |
32000 | - |
1 * 29000, A-chain, + 1 * 32000, B-chain, SDS-PAGE | Aralia elata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Aralia elata | the cytotoxic enzyme induces apoptosis in transformed cells by inhibiting protein synthesis and inactivating ribosomes, the enzyme targets diverse cells, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aralia elata | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Aralia elata |
Purification (Comment) | Organism |
---|---|
native enzyme 611.4fold from shoots by ammonium sulfate fractionation, ion exchange chromatography and gel filtration | Aralia elata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
shoot | - |
Aralia elata | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
cytotoxic effects on WI-38 and VA-13 cells | Aralia elata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
28S rRNA + H2O | substrate from rat liver ribosomes, generation of an R-fragment from 28S RNA, product determination using aniline coupling | Aralia elata | ? | - |
? | |
additional information | the cytotoxic enzyme induces apoptosis in transformed cells by inhibiting protein synthesis and inactivating ribosomes, the enzyme targets diverse cells, overview | Aralia elata | ? | - |
? | |
additional information | the enzyme targets diverse cells, e.g. WI-38 cells or VA-13 cells, overview | Aralia elata | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 29000, A-chain, + 1 * 32000, B-chain, SDS-PAGE | Aralia elata |
Synonyms | Comment | Organism |
---|---|---|
aralin | - |
Aralia elata |
RIP | - |
Aralia elata |
RNA N-glycosidase | - |
Aralia elata |
type II ribosome-inactivating protein | - |
Aralia elata |