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Literature summary for 3.2.2.22 extracted from
- Ricin A-chain substrate specificity in RNA, DNA, and hybrid stem-loop structures (2004), Biochemistry, 43, 4913-4922.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of ricin A-chain RTA V81M without N- and C-terminal extensions in Escherichia coli strain BL21(DE3) | Ricinus communis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics with RNA, DNA, and hybrid stem-loop substrates, overview | Ricinus communis | |
| 0.0007 | - |
5'-G1C2G3C4dG5A6G7A8G9C10G11C12-3' | 37°C | Ricinus communis |  |
| 0.0009 | - |
5'-G1C2G3C4G5A6dG7A8G9C10G11C12-3' | 37°C | Ricinus communis | |
| 0.0018 | - |
5'-G1C2G3C4dG5A6G7dA8G9C10G11C12-3' | 37°C | Ricinus communis | |
| 0.0027 | - |
5'-G1C2G3C4G5A6G7A8G9C10G11C12-3' | 37°C | Ricinus communis | |
| 0.0029 | - |
5'-G1C2G3C4mG5A6G7A8G9C10G11C12-3' | 37°C | Ricinus communis | |
| 0.005 | - |
5'-dG1dC2dG3dC4dG5dA6dG7A8dG9dC10dG11dC12-3' | 37°C | Ricinus communis | |
| 0.0057 | - |
5'-G1C2G3C4G5A6G7dA8G9C10G11C12-3' | 37°C | Ricinus communis | |
| 0.0063 | - |
5'-G1C2G3C4G5A6mG7A8G9C10G11C12-3' | 37°C | Ricinus communis | |
| 0.0094 | - |
5'-dG1dC2dG3dC4G5dA6dG7dA8dG9dC10dG11dC12-3' | 37°C | Ricinus communis | |
| 0.0097 | - |
5'-G1C2G3C4G5A6G7mA8G9C10G11C12-3' | 37°C | Ricinus communis | |
| 0.01 | - |
5'-dG1dC2dG3dC4dG5A6dG7dA8dG9dC10dG11dC12-3' | 37°C | Ricinus communis | |
| 0.0152 | - |
5'-dG1dC2dG3dC4dG5dA6G7dA8dG9dC10dG11dC12-3' | 37°C | Ricinus communis | |
| 0.032 | - |
5'-G1C2G3C4G5dA6G7A8G9C10G11C12-3' | 37°C | Ricinus communis | |
| 0.1 | - |
5'-G1C2G3C4dG5A6dG7A8G9C10G11C12-3' | 37°C | Ricinus communis | |
| 0.3 | - |
5'-G1C2G3C4G5mA6G7A8G9C10G11C12-3' | 37°C | Ricinus communis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ricinus communis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant ricin A-chain RTA V81M without N- and C-terminal extensions from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation and anion exchange chromatography | Ricinus communis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| A4324 in 28S rRNA + H2O = deadenylated 28S rRNA + adenine | catalytic mechanism | Ricinus communis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Ricinus communis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 28S rRNA + H2O | in ribosomes, generation of an R-fragment from 28S RNA, depurination of A4324 | Ricinus communis | ? | - |
? | |
| 5'-dG1dC2dG3dC4dG5A6dG7dA8dG9dC10dG11dC12-3' + H2O | GAGA stem-loop RNA-DNA hybrid substrate, analysis of activity with substrate derivative possessing variations in the tetraloop G5-A8, overview | Ricinus communis | ? | - |
? | |
| 5'-dG1dC2dG3dC4dG5dA6dG7A8dG9dC10dG11dC12-3' + H2O | GAGA stem-loop RNA-DNA hybrid substrate, analysis of activity with substrate derivative possessing variations in the tetraloop G5-A8, overview | Ricinus communis | ? | - |
? | |
| 5'-dG1dC2dG3dC4dG5dA6G7dA8dG9dC10dG11dC12-3' + H2O | GAGA stem-loop RNA-DNA hybrid substrate, analysis of activity with substrate derivative possessing variations in the tetraloop G5-A8, overview | Ricinus communis | ? | - |
? | |
| 5'-dG1dC2dG3dC4G5dA6dG7dA8dG9dC10dG11dC12-3' + H2O | GAGA stem-loop RNA-DNA hybrid substrate, analysis of activity with substrate derivative possessing variations in the tetraloop G5-A8, overview | Ricinus communis | ? | - |
? | |
| 5'-G1C2G3C4dG5A6dG7A8G9C10G11C12-3' + H2O | GAGA stem-loop RNA-DNA hybrid substrate, analysis of activity with substrate derivative possessing variations in the tetraloop G5-A8, overview | Ricinus communis | ? | - |
? | |
| 5'-G1C2G3C4dG5A6G7A8G9C10G11C12-3' + H2O | GAGA stem-loop RNA-DNA hybrid substrate, analysis of activity with substrate derivative possessing variations in the tetraloop G5-A8, overview | Ricinus communis | ? | - |
? | |
| 5'-G1C2G3C4dG5A6G7dA8G9C10G11C12-3' + H2O | GAGA stem-loop RNA-DNA hybrid substrate, analysis of activity with substrate derivative possessing variations in the tetraloop G5-A8, overview | Ricinus communis | ? | - |
? | |
| 5'-G1C2G3C4G5A6dG7A8G9C10G11C12-3' + H2O | GAGA stem-loop RNA-DNA hybrid substrate, analysis of activity with substrate derivative possessing variations in the tetraloop G5-A8, overview | Ricinus communis | ? | - |
? | |
| 5'-G1C2G3C4G5A6G7A8G9C10G11C12-3' + H2O | GAGA stem-loop RNA substrate, analysis of activity with substrate derivative possessing variations in the tetraloop G5-A8, preferred substrates to DNA stem loop derivatives, overview | Ricinus communis | ? | - |
? | |
| 5'-G1C2G3C4G5A6G7dA8G9C10G11C12-3' + H2O | GAGA stem-loop RNA-DNA hybrid substrate, analysis of activity with substrate derivative possessing variations in the tetraloop G5-A8, overview | Ricinus communis | ? | - |
? | |
| 5'-G1C2G3C4G5A6G7mA8G9C10G11C12-3' + H2O | RNA-2'-methoxy nucleic acid hybrid stem-loop substrate | Ricinus communis | ? | - |
? | |
| 5'-G1C2G3C4G5A6mG7A8G9C10G11C12-3' + H2O | RNA-2'-methoxy nucleic acid hybrid stem-loop substrate | Ricinus communis | ? | - |
? | |
| 5'-G1C2G3C4G5dA6G7A8G9C10G11C12-3' + H2O | GAGA stem-loop RNA-DNA hybrid substrate, analysis of activity with substrate derivative possessing variations in the tetraloop G5-A8, overview | Ricinus communis | ? | - |
? | |
| 5'-G1C2G3C4G5mA6G7A8G9C10G11C12-3' + H2O | RNA-2'-methoxy nucleic acid hybrid stem-loop substrate | Ricinus communis | ? | - |
? | |
| 5'-G1C2G3C4mG5A6G7A8G9C10G11C12-3' + H2O | RNA-2'-methoxy nucleic acid hybrid stem-loop substrate | Ricinus communis | ? | - |
? | |
| additional information | substrate specificity of catalytic ricin A-chain with RNA, DNA, and hybrid stem-loop structures | Ricinus communis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | heterodimer formed by a catalytic subunit A, RTA, and a carbohydrate binding subunit B, RTB | Ricinus communis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ricin | - |
Ricinus communis |
| type II ribosome-inactivating protein | - |
Ricinus communis |
| type II RIP | - |
Ricinus communis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Ricinus communis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0007 | - |
5'-G1C2G3C4dG5A6dG7A8G9C10G11C12-3' | 37°C | Ricinus communis | |
| 0.001 | - |
5'-G1C2G3C4mG5A6G7A8G9C10G11C12-3' | 37°C | Ricinus communis | |
| 0.0015 | - |
5'-dG1dC2dG3dC4dG5dA6G7dA8dG9dC10dG11dC12-3' | 37°C | Ricinus communis | |
| 0.0038 | - |
5'-dG1dC2dG3dC4G5dA6dG7dA8dG9dC10dG11dC12-3' | 37°C | Ricinus communis | |
| 0.005 | - |
5'-G1C2G3C4dG5A6G7A8G9C10G11C12-3' | 37°C | Ricinus communis | |
| 0.005 | - |
5'-G1C2G3C4G5A6mG7A8G9C10G11C12-3' | 37°C | Ricinus communis | |
| 0.0083 | - |
5'-dG1dC2dG3dC4dG5A6dG7dA8dG9dC10dG11dC12-3' | 37°C | Ricinus communis | |
| 0.01 | - |
5'-G1C2G3C4dG5A6G7dA8G9C10G11C12-3' | 37°C | Ricinus communis | |
| 0.011 | - |
5'-G1C2G3C4G5A6dG7A8G9C10G11C12-3' | 37°C | Ricinus communis | |
| 0.015 | - |
5'-dG1dC2dG3dC4dG5dA6dG7A8dG9dC10dG11dC12-3' | 37°C | Ricinus communis | |
| 0.055 | - |
5'-G1C2G3C4G5A6G7dA8G9C10G11C12-3' | 37°C | Ricinus communis | |
| 0.07 | - |
5'-G1C2G3C4G5mA6G7A8G9C10G11C12-3' | 37°C | Ricinus communis | |
| 0.085 | - |
5'-G1C2G3C4G5A6G7mA8G9C10G11C12-3' | 37°C | Ricinus communis | |
| 1.68 | - |
5'-G1C2G3C4G5A6G7A8G9C10G11C12-3' | 37°C | Ricinus communis | |
| 27.67 | - |
5'-G1C2G3C4G5dA6G7A8G9C10G11C12-3' | 37°C | Ricinus communis | |
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