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Literature summary for 3.2.2.22 extracted from
- Purification of a new ribosome-inactivating protein from the seeds of Cinnamomum porrectum and characterization of the RNA N-glycosidase activity of the toxic protein (1996), Biol. Chem., 377, 825-831.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30500 | - |
1 * 30500 + 1 * 33500, A-chain and B-chain, SDS-PAGE | Cinnamomum parthenoxylon |
| 33500 | - |
1 * 30500 + 1 * 33500, A-chain and B-chain, SDS-PAGE | Cinnamomum parthenoxylon |
| 58000 | - |
gel filtration | Cinnamomum parthenoxylon |
| 64500 | - |
- |
Cinnamomum parthenoxylon |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| rRNA + H2O | Cinnamomum parthenoxylon | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cinnamomum parthenoxylon | - |
camphor tree | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | porrectin | Cinnamomum parthenoxylon |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cinnamomum parthenoxylon |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Cinnamomum parthenoxylon | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific activity 0.0215 units/mg, 1 unit is the amount of protein necessary to inhibit protein synthesis by 50% in 0.1 ml | Cinnamomum parthenoxylon |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | damages eukaryotic ribosomes by cleaving the N-glycosidic bond at A4324 of the 28 S rRNA | Cinnamomum parthenoxylon | ? | - |
? | |
| rRNA + H2O | - |
Cinnamomum parthenoxylon | ? | - |
? | |
| rRNA + H2O | cleaves the N-glycosidic bond and removes adenine at A-4324 in 28 S rRNA when intact rat ribosomes are the substrate, cleaves the same N-glycosidic bond in naked 28 S rRNA | Cinnamomum parthenoxylon | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 30500 + 1 * 33500, A-chain and B-chain, SDS-PAGE | Cinnamomum parthenoxylon |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| porrectin | - |
Cinnamomum parthenoxylon |
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