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Literature summary for 3.2.2.22 extracted from
- Purification and properties of a new ribosome-inactivating protein with RNA N-glycosidase activity suitable for immunotoxin preparation from the seeds of Momordica cochinchinensis (1989), Biochim. Biophys. Acta, 993, 287-292.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30700 | - |
1 * 30700, momorchochin-S, SDS-PAGE | Momordica cochinchinensis |
| 31000 | - |
momorchochin-S, gel filtration | Momordica cochinchinensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| rRNA + H2O | Momordica cochinchinensis | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Momordica cochinchinensis | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Momordica cochinchinensis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Momordica cochinchinensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Momordica cochinchinensis | - |
| seed | - |
Momordica cochinchinensis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
0.2857 units/mg, one unit is the amount of protein necessary to inhibit protein synthesis by 50% in 1 ml rabbit reticulocyte lysate | Momordica cochinchinensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | inhibits protein synthesis by a rabbit-reticulocyte lysate and phenylalanine polymerization by isolated ribosomes | Momordica cochinchinensis | ? | - |
? | |
| additional information | momorcochin-S damages rRNA in the same manner as ricin | Momordica cochinchinensis | ? | - |
? | |
| rRNA + H2O | - |
Momordica cochinchinensis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 30700, momorchochin-S, SDS-PAGE | Momordica cochinchinensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| momorcochin | isoform purified from roots | Momordica cochinchinensis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Momordica cochinchinensis | alkaline isoelectric point | - |
additional information |
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Release 2023.1 (January 2023)
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