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Literature summary for 3.2.2.21 extracted from

  • Bowman, B.R.; Lee, S.; Wang, S.; Verdine, G.L.
    Structure of Escherichia coli AlkA in complex with undamaged DNA (2010), J. Biol. Chem., 285, 35783-35791.
    View publication on PubMedView publication on EuropePMC
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Crystallization (Commentary)

Crystallization (Comment) Organism
AlkA in complex with undamaged DNA, sitting drop vapor diffusion method, using 25-29% (w/v) polyethylene glycol 3350, 100 mM bis-Tris, pH 6.0-6.6, 200 mM Li2S04, and 3% (w/v) 6-aminocaproic acid, at 25°C Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
2'-deoxy-2'-fluoro-7-methylguanosine
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P04395
-
-

Purification (Commentary)

Purification (Comment) Organism
Mono Q column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alkylated DNA + H2O the sequences of the oligos used in the assay are 5'-CGATAGCATCCTYCCTTCTCTCCAT-3', where Y is the location of the lesion base, and 5'-ATGGAGAGAAGGZAGGATGCTATCG-3' for the complementary strand, where Z is the base opposite the lesion Escherichia coli 3-methyl-2'-deoxyadenosine + 3-deaza-3-methyl-2'-deoxyadenosine + DNA
-
 ?
alkylated DNA + H2O
-
 Escherichia coli 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + 1,N6-ethenoadenine + hypoxanthine + DNA
-
 ?
additional information the enzyme has little activity on correctly base-paired adenine. N3-deazaadenine is not substantially cleaved opposite cytosine or thymidine Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
AlkA
-
 Escherichia coli