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Literature summary for 3.2.2.21 extracted from
- Expression, purification and characterization of codon-optimized human N-methylpurine-DNA glycosylase from Escherichia coli (2008), Protein Expr. Purif., 58, 257-262.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000053 | - |
hypoxanthine | at 37°C | Homo sapiens |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | optimal activity at pH 7.5 and 100 mM KCl | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| SP Sepharose column chromatography | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the crude extract shows a specific activity of 80 IU/mg, the 125fold purified enzyme displays a specific activity of 10000 IU/mg, one international unit (IU) is defined as the activity, which cleaves 50% of 0.5 pmole of double stranded DNA oligo substrate containing a hypoxanthine lesion in 10 min at 37°C | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alkylated DNA + H2O | - |
Homo sapiens | 3-methyladenine + 7-methylguanine + hypoxanthine + DNA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MPG | - |
Homo sapiens |
| N-methylpurine-DNA glycosylase | - |
Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
the purified full-length MPG is stable at high temperature, at 50 °C 30 min of incubation inactivate the protein by 30% | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0033 | - |
hypoxanthine | at 37°C | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Homo sapiens |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Homo sapiens | isoelectric focusing | - |
9.65 |
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