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Literature summary for 3.2.2.21 extracted from

  • Roy, R.; Biswas, T.; Hazra, T.K.; Roy, G.; Grabowski, D.T.; Izumi, T.; Srinivasan, G.; Mitra, S.
    Specific interaction of wild-type and truncated mouse N-methylpurine-DNA glycosylase with ethenoadenine-containing DNA (1998), Biochemistry, 37, 580-589.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
truncated MPG polypeptides expressed in Escherichia coli using vector pRSET, full length MPG protein cloned in eukaryotic baculovirus expression vector pVL1393 and host Sf9 insect cells used for expression Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00004 0.001 1,N6-ethenoadenine residues in alkylated DNA pH 8.3, 37°C, release of 1,N6-ethenoadenine, wild type enzyme Mus musculus
0.00006 0.0013 1,N6-ethenoadenine residues in alkylated DNA pH 8.3, 37°C, release of 1,N6-ethenoadenine, truncated mutant N-DELTA100C-DELTA18 Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alkylated DNA + H2O Mus musculus
-
3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
mouse
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, expressed from baculovirus Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,N6-ethenoadenine residues in alkylated DNA + H2O
-
Mus musculus ethenoadenine + DNA
-
?
alkylated DNA + H2O
-
Mus musculus 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
?
additional information removes also N-alkylpurines and cyclic ethenoadducta of adenine, guanine and cytosine Mus musculus ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
1,N6-ethenoadenine residues in alkylated DNA pH 8.3, 37°C, release of 1,N6-ethenoadenine, truncated mutant N-DELTA100C-DELTA18 Mus musculus
0.0167
-
1,N6-ethenoadenine residues in alkylated DNA pH 8.3, 37°C, release of 1,N6-ethenoadenine, wild-type Mus musculus