Cloned (Comment) | Organism |
---|---|
truncated MPG polypeptides expressed in Escherichia coli using vector pRSET, full length MPG protein cloned in eukaryotic baculovirus expression vector pVL1393 and host Sf9 insect cells used for expression | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00004 | 0.001 | 1,N6-ethenoadenine residues in alkylated DNA | pH 8.3, 37°C, release of 1,N6-ethenoadenine, wild type enzyme | Mus musculus | |
0.00006 | 0.0013 | 1,N6-ethenoadenine residues in alkylated DNA | pH 8.3, 37°C, release of 1,N6-ethenoadenine, truncated mutant N-DELTA100C-DELTA18 | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alkylated DNA + H2O | Mus musculus | - |
3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
mouse | - |
Purification (Comment) | Organism |
---|---|
recombinant protein, expressed from baculovirus | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,N6-ethenoadenine residues in alkylated DNA + H2O | - |
Mus musculus | ethenoadenine + DNA | - |
? | |
alkylated DNA + H2O | - |
Mus musculus | 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA | - |
? | |
additional information | removes also N-alkylpurines and cyclic ethenoadducta of adenine, guanine and cytosine | Mus musculus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
1,N6-ethenoadenine residues in alkylated DNA | pH 8.3, 37°C, release of 1,N6-ethenoadenine, truncated mutant N-DELTA100C-DELTA18 | Mus musculus | |
0.0167 | - |
1,N6-ethenoadenine residues in alkylated DNA | pH 8.3, 37°C, release of 1,N6-ethenoadenine, wild-type | Mus musculus |