Literature summary for 3.2.2.21 extracted from

Yamagata, Y.; Kato, M.; Odawara, K.; Tokuno, Y.; Nakashima, Y.; Matsushima, N.; Yasumura, K.; Tomita, K.; Ihara, K.; Fujii, Y.; Nakabeppu, Y.; Sekiguchi, M.; Fujii, S.
Three-dimensional structure of a DNA repair enzyme, 3-methyladenine DNA glycosylase II, from Escherichia coli (1996), Cell, 86, 311-319.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure of AlkA solved with the multiple isomorphous replacement method, crystals grown with sitting-drop vapor-diffusion technique, space group P2(1), cell dimensions a = 58.61 A, b = 76.93 A, c = 62.27 A	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
D238N	site-directed mutagenesis	Escherichia coli
W218A	site-directed mutagenesis	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
alkylated DNA + H2O	Escherichia coli	-	3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
purified from overproducing strains DH1 and HB101, harboring plasmid pYN3070	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alkylated DNA + H2O	-	Escherichia coli	3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA	-	?

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Release 2023.1 (January 2023)