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Literature summary for 3.2.2.21 extracted from

  • Bjelland, S.; Birkeland, N.K.; Benneche, T.; Volden, G.; Seeberg, E.
    DNA glycosylase activities for thymine residues oxidized in the methyl group are functions of the AlkA enzyme in Escherichia coli (1994), J. Biol. Chem., 269, 30489-30495.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00000014
-
alkylated DNA pH 7.5, 37°C, excision of 5-hydroxymethyluracil, denatured DNA Escherichia coli
0.0000044
-
alkylated DNA pH 7.5, 37°C, excision of 5-hydroxymethyluracil Escherichia coli
0.0000108
-
alkylated DNA pH 7.5, 37°C, excision of 3-methyladenine Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alkylated DNA + H2O Escherichia coli DNA repair enzyme, inducible base excision repair pathway of DNA alkylation damage 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0247
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
-70°C, purified enzyme remains stable for more than 4 years when stored in 20% glycerol, Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alkylated DNA + H2O
-
Escherichia coli 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
?
alkylated DNA + H2O DNA repair enzyme, inducible base excision repair pathway of DNA alkylation damage Escherichia coli 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
?
additional information enzyme also excises thymine residues oxidized in the methyl group like 5-formyluracil and 5-hydroxymethyluracil Escherichia coli ?
-
?