Crystallization (Comment) | Organism |
---|---|
structure resembling a catalytic intermediate complex. AlkC uses unique HEAT-like repeat folds and immunoglobulin-like domains to induce a sharp kink in the DNA, exposing the damaged nucleobase to active site residues that project into the DNA. The active site can accommodate and excise N3-methylcytosine and N1-methyladenine | Pseudomonas fluorescens |
structure resembling a catalytic intermediate complex. AlkC uses unique HEAT-like repeat folds and immunoglobulin-like domains to induce a sharp kink in the DNA, exposing the damaged nucleobase to active site residues that project into the DNA. The active site can accommodate and excise N3-methylcytosine and N1-methyladenine | Bacillus cereus |
Protein Variants | Comment | Organism |
---|---|---|
E121A | about 20% of wild-type activity | Pseudomonas fluorescens |
E156A | about 50% of wild-type activity | Pseudomonas fluorescens |
W164A | activity similar to wild-type | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Pseudomonas fluorescens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alkylated DNA + H2O | - |
Pseudomonas fluorescens | 3-methyladenine + ? | - |
? | |
alkylated DNA + H2O | - |
Bacillus cereus | 3-methyladenine + ? | - |
? | |
alkylated DNA + H2O | - |
Pseudomonas fluorescens | 1-methyladenine + ? | - |
? | |
alkylated DNA + H2O | - |
Bacillus cereus | 1-methyladenine + ? | - |
? | |
alkylated DNA + H2O | - |
Pseudomonas fluorescens | 3-methylcytosine + ? | - |
? | |
alkylated DNA + H2O | - |
Bacillus cereus | 3-methylcytosine + ? | - |
? | |
additional information | AlkC exhibits robust activity for 3-methylcytosine and modest activity for 1-methyladenosine | Pseudomonas fluorescens | ? | - |
? | |
additional information | AlkC exhibits robust activity for 3-methylcytosine and modest activity for 1-methyladenosine | Bacillus cereus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AlkC | - |
Pseudomonas fluorescens |
AlkC | - |
Bacillus cereus |