BRENDA - Enzyme Database show
show all sequences of 3.2.2.20

Solution structure and base perturbation studies reveal a novel mode of alkylated base recognition by 3-methyladenine DNA glycosylase I

Cao, C.; Kwon, K.; Jiang, Y.L.; Drohat, A.C.; Stivers, J.T.; J. Biol. Chem. 278, 48012-48020 (2003)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
D18A
TAG mutant, prepared with QuikChange double-stranded mutagenesis kit
Escherichia coli
E38A
TAG mutant, prepared with QuikChange double-stranded mutagenesis kit
Escherichia coli
H17A
TAG mutant, prepared with QuikChange double-stranded mutagenesis kit
Escherichia coli
W21A
TAG mutant, prepared with QuikChange double-stranded mutagenesis kit
Escherichia coli
W46A
TAG mutant, prepared with QuikChange double-stranded mutagenesis kit
Escherichia coli
Y13A
TAG mutant, prepared with QuikChange double-stranded mutagenesis kit
Escherichia coli
Y16A
TAG mutant, prepared with QuikChange double-stranded mutagenesis kit
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alkylated DNA + H2O
Escherichia coli
-
3-methyladenine + ?
-
Escherichia coli
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alkylated DNA + H2O
-
646863
Escherichia coli
3-methyladenine + ?
-
646863
Escherichia coli
?
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D18A
TAG mutant, prepared with QuikChange double-stranded mutagenesis kit
Escherichia coli
E38A
TAG mutant, prepared with QuikChange double-stranded mutagenesis kit
Escherichia coli
H17A
TAG mutant, prepared with QuikChange double-stranded mutagenesis kit
Escherichia coli
W21A
TAG mutant, prepared with QuikChange double-stranded mutagenesis kit
Escherichia coli
W46A
TAG mutant, prepared with QuikChange double-stranded mutagenesis kit
Escherichia coli
Y13A
TAG mutant, prepared with QuikChange double-stranded mutagenesis kit
Escherichia coli
Y16A
TAG mutant, prepared with QuikChange double-stranded mutagenesis kit
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alkylated DNA + H2O
Escherichia coli
-
3-methyladenine + ?
-
Escherichia coli
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alkylated DNA + H2O
-
646863
Escherichia coli
3-methyladenine + ?
-
646863
Escherichia coli
?
Other publictions for EC 3.2.2.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732176
Liu
A TetR family transcriptional ...
Mycobacterium tuberculosis variant bovis
J. Biol. Chem.
289
9065-9075
2014
1
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1
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732554
Troll
Interplay between base excisio ...
Saccharomyces cerevisiae, Saccharomyces pombe
Mutat. Res.
763-764
64-73
2014
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2
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732445
Rajesh
-
Cheminformatic designing of de ...
Leptospira interrogans
Med. Chem. Res.
22
3434-3443
2013
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1
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15
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2
1
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732589
Finney-Manchester
Harnessing mutagenic homologou ...
Saccharomyces cerevisiae
Nucleic Acids Res.
41
e99
2013
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731077
Zhu
A model for 3-methyladenine re ...
Staphylococcus aureus, Staphylococcus aureus MSSA476
Acta Crystallogr. Sect. F
68
610-615
2012
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1
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731324
Yang
Physical and functional intera ...
Mycobacterium tuberculosis, Mycolicibacterium smegmatis
Biochemistry
77
378-387
2012
-
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2
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2
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6
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2
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2
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2
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732942
Hasplova
DNA alkylation lesions and the ...
Homo sapiens
Toxicol. Lett.
208
76-81
2012
-
-
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2
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3
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1
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1
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2
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707488
Lee
Recognition and processing of ...
Homo sapiens
Biochemistry
48
1850-1861
2009
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1
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1
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2
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1
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1
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2
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1
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1
1
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697578
Lingaraju
Substrate specificity and sequ ...
Saccharomyces cerevisiae
DNA Repair
7
970-982
2008
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2
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2
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679500
Metz
DNA damage recognition and rep ...
Salmonella enterica subsp. enterica serovar Typhimurium
EMBO J.
26
2411-2420
2007
-
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1
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3
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698111
Kanamitsu
Involvement of 3-methyladenine ...
Schizosaccharomyces pombe
Genes Genet. Syst.
82
489-494
2007
-
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1
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5
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1
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670318
Alseth
A new protein superfamily incl ...
Bacillus cereus
Mol. Microbiol.
59
1602-1609
2006
-
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1
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1
1
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6
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1
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3
1
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1
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1
1
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1
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3
1
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646865
Aamodt
The Bacillus subtilis counterp ...
Bacillus subtilis, Bacillus subtilis 168, Homo sapiens
J. Biol. Chem.
279
13601-13606
2004
-
-
1
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3
-
89
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2
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5
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5
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646866
Likhite
Interaction of estrogen recept ...
Homo sapiens
J. Biol. Chem.
279
16875-16882
2004
-
-
1
-
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-
-
-
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1
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1
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2
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2
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1
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2
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646863
Cao
Solution structure and base pe ...
Escherichia coli
J. Biol. Chem.
278
48012-48020
2003
-
-
-
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7
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1
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1
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1
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7
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1
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1
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646864
Kwon
A novel zinc snap motif convey ...
Escherichia coli
J. Biol. Chem.
278
19442-19446
2003
-
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1
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1
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1
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2
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1
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2
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646860
Bujnicki
Fold-recognition analysis pred ...
Escherichia coli, Homo sapiens
DNA Repair
1
391-395
2002
-
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2
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3
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2
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646861
Drohat
3-Methyladenine DNA glycosylas ...
Escherichia coli, Escherichia coli B / ATCC 11303
Nat. Struct. Biol.
9
659-664
2002
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1
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1
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5
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646862
Hendricks
The S. cerevisiae Mag1 3-methy ...
Saccharomyces cerevisiae
DNA Repair
1
645-659
2002
-
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1
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4
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646857
Plochocka
3-Methyladenine-DNA glycosylas ...
Escherichia coli
Biochem. Biophys. Res. Commun.
268
724-727
2000
-
-
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1
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646858
Smith
In vivo repair of methylation ...
Mus musculus
Nucleic Acids Res.
28
3294-3300
2000
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1
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646859
Wyatt
Influence of DNA structure on ...
Mus musculus
Carcinogenesis
21
901-908
2000
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1
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646855
Bjelland
Different efficiencies of the ...
Escherichia coli
FEBS Lett.
397
127-129
1996
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646856
Tomicic
Effect of overexpression of E. ...
Escherichia coli
Mutat. Res.
358
81-87
1996
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646854
Seeberg
Physical and genetic mapping o ...
Escherichia coli
J. Bacteriol.
175
5733-5734
1993
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1
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1
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1
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1
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1
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1
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-
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1
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-
646852
Klungland
Expression of the E.coli 3-met ...
Escherichia coli
EMBO J.
11
4439-4444
1992
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-
1
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1
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5
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1
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1
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1
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-
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1
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-
646853
Taverna
Expression of E. coli tag gene ...
Escherichia coli
Biochem. Biophys. Res. Commun.
185
41-46
1992
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-
1
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1
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1
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2
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1
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1
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2
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-
646850
Bjelland
Purification and characterizat ...
Escherichia coli
Nucleic Acids Res.
15
2787-2801
1987
3
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-
1
3
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3
2
1
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3
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1
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1
2
2
1
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1
1
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3
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1
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3
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3
2
1
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1
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1
2
2
1
-
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-
-
1
1
-
-
-
-
-
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-
646851
Riazuddin
DNA glycosylase enzymes induce ...
Escherichia coli, Micrococcus luteus
Nucleic Acids Res.
15
6607-6624
1987
-
-
-
-
-
1
8
-
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4
2
3
-
2
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1
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1
1
7
-
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3
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2
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2
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1
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8
-
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4
2
3
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1
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1
1
7
-
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3
-
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-
646849
Steinum
Nucleotide sequence of the tag ...
Escherichia coli
Nucleic Acids Res.
14
3763-3772
1986
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1
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-
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1
1
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2
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2
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1
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1
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1
1
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2
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1
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646848
Clarke
Cloning of Escherichia coli ge ...
Escherichia coli
Mol. Gen. Genet.
197
368-372
1984
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1
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2
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2
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3
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1
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2
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3
-
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-
-
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646845
Evensen
Adaptation to alkylation resis ...
Escherichia coli
Nature
296
773-775
1982
-
-
-
-
-
-
-
-
-
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-
1
-
1
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1
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1
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-
1
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646846
Karran
Induction of a DNA glycosylase ...
Escherichia coli, no activity in Escherichia coli
Nature
296
770-773
1982
-
-
-
-
-
-
-
-
-
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1
1
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2
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2
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1
1
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-
-
2
-
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646847
Thomas
Two DNA glycosylases in Escher ...
Escherichia coli, Escherichia coli BW 9062, no activity in Escherichia coli
Biochemistry
21
1162-1169
1982
-
-
-
-
-
-
6
1
-
1
1
2
-
3
-
-
1
-
-
-
-
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6
-
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1
-
1
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1
1
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6
1
1
-
1
1
2
-
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1
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-
6
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
646844
Riazuddin
Properties of 3-methyladenine- ...
Escherichia coli
Biochemistry
17
2110-2118
1978
-
-
-
-
-
-
2
1
-
2
1
2
-
1
-
-
1
-
-
-
1
1
4
-
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-
1
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-
1
-
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-
1
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-
-
-
2
-
1
-
2
1
2
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1
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1
1
4
-
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1
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