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Literature summary for 3.2.2.2 extracted from

  • Szuwart, M.; Starzynska, E.; Pietrowska-Borek, M.; Guranowski, A.
    Calcium-stimulated guanosine--inosine nucleosidase from yellow lupin (Lupinus luteus) (2006), Phytochemistry, 67, 1476-1485.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2'-deoxyguanosine competitive Lupinus luteus
2'-methylguanosine competitive Lupinus luteus
adenosine competitive Lupinus luteus
guanine competitive Lupinus luteus
Mn2+
-
Lupinus luteus
Zn2+
-
Lupinus luteus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
Inosine pH 4.8 Lupinus luteus
0.0027
-
guanosine pH 4.8 Lupinus luteus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ up to 6fold activation by exogenous CaCl2 or Ca(NO3)2 Lupinus luteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
gel filtration Lupinus luteus

Organism

Organism UniProt Comment Textmining
Lupinus luteus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Lupinus luteus

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon 5fold increase in activity during seed germination Lupinus luteus
-
seed
-
Lupinus luteus
-
seedling
-
Lupinus luteus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.25
-
pH 4.8 Lupinus luteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-methylguanosine + H2O
-
Lupinus luteus 1-methylguanine + D-ribose
-
?
1-N2-ethenoguanosine + H2O
-
Lupinus luteus 1-N2-ethenoguanine + D-ribose
-
?
1-N2-isopropenoguanosine + H2O
-
Lupinus luteus 1-N2-isopropenoguanine + D-ribose
-
?
7-methylguanosine + H2O
-
Lupinus luteus 7-methylguanine + D-ribose
-
?
guanosine + H2O guanosine is hydrolyzed 12% faster than inosine Lupinus luteus guanine + D-ribose
-
?
inosine + H2O guanosine is hydrolyzed 12% faster than inosine Lupinus luteus hypoxanthine + D-ribose
-
?
additional information adenosine and xanthosine are poor substrates. No substrate are: 2'-deoxyguanosine, 2'-deoxyinosine, 2'-methylguanosine, pyrimidine nucleosides and 5'-GMP Lupinus luteus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 80000, SDS-PAGE Lupinus luteus

Synonyms

Synonyms Comment Organism
guanosine-inosine-preferring nucleoside N-ribohydrolase
-
Lupinus luteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.7 5.5
-
Lupinus luteus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0015
-
pH 4.8 Lupinus luteus 2'-deoxyguanosine
0.0036
-
pH 4.8 Lupinus luteus 2'-methylguanosine
0.0097
-
pH 4.8 Lupinus luteus guanine
0.021
-
pH 4.8 Lupinus luteus adenosine
0.06
-
pH 4.8 Lupinus luteus Mn2+
0.06
-
pH 4.8 Lupinus luteus Zn2+