Literature summary for 3.2.2.19 extracted from

Kernstock, S.; Koch-Nolte, F.; Mueller-Dieckmann, J.; Weiss, M.S.; Mueller-Dieckmann, C.
Cloning, expression, purification and crystallization as well as X-ray fluorescence and preliminary X-ray diffraction analyses of human ADP-ribosylhydrolase 1 (2009), Acta Crystallogr. Sect. F, 65, 529-532.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of His-tagged hARH1 in Escherichia coli strain BL21(DE3)	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant hARH1 in complex with K+ and ADP, mixing of equal volumes of protein solution containing 15-20 mg/ml protein in 20 mM HEPES, pH 7.0, 100 mM NaCl and 3 mM DTT with reservoir solution containing 150 mM lithium acetate and 25% w/v PEG 3350, at 20°C, equilibration over 0.75 ml reservoir solution, 2 weeks, X-ray diffraction structure determination and analysis at 1.9 A resolution	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	ADPRH cleaves the glycosidic bond of ADP-ribose attached to an Arg residue of a protein	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P54944	hARH1	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged hARH1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	ADPRH cleaves the glycosidic bond of ADP-ribose attached to an Arg residue of a protein	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
ADP-ribosylhydrolase 1	-	Homo sapiens
ADPRH	-	Homo sapiens
hARH1	-	Homo sapiens

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Release 2023.1 (January 2023)