Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | enhances, activation is maximal with 5-10 mM dithiothreitol and 5-10 mM MgCl2 | Meleagris gallopavo | |
dithiothreitol | synergistically stimulated by Mg2+ and dithiothreitol | Mus musculus | |
dithiothreitol | synergistically stimulated by Mg2+ and dithiothreitol | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
expression as a glutathione S-transferase-linked fusion protein in Escherichia coli | Rattus norvegicus |
expression in Escherichia coli | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | inhibition by ADP stronger than by AMP | Meleagris gallopavo | |
ADP | - |
Rattus norvegicus | |
ADPribose | inhibition by ADPribose stronger than by ADP and AMP | Meleagris gallopavo | |
ADPribose | - |
Oryctolagus cuniculus | |
ADPribose | - |
Rattus norvegicus | |
AMP | - |
Meleagris gallopavo | |
NaCl | inhibition with 200 mM | Meleagris gallopavo | |
NaF | more than 80% inhibition with 5 mM | Meleagris gallopavo |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Meleagris gallopavo | |
Mg2+ | - |
Cavia porcellus | |
Mg2+ | - |
Mus musculus | |
Mg2+ | - |
Rattus norvegicus | |
Mg2+ | - |
Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
gel filtration, SDS-PAGE | Meleagris gallopavo |
39000 | - |
immunoblot with polyclonal rabbit anti-rat brain hydrolase antibodies | Meleagris gallopavo |
39000 | - |
immunoblot with polyclonal rabbit anti-rat brain hydrolase antibodies | Mus musculus |
39000 | - |
immunoblot with polyclonal rabbit anti-rat brain hydrolase antibodies | Rattus norvegicus |
39000 | - |
immunoblot with polyclonal rabbit anti-rat brain hydrolase antibodies | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-ribose-arginine + H2O | Meleagris gallopavo | - |
ADP-ribose + arginine | - |
? | |
ADP-ribose-arginine + H2O | Rhodospirillum rubrum | the existence of a ADP-ribosylation cycle was established in the bacterium Rhodospirillium rubrum where it regulates dinitrogenase reductase, a key enzyme in nitrogen fixation | ADP-ribose + arginine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Cavia porcellus | - |
- |
- |
Meleagris gallopavo | - |
- |
- |
Mus musculus | - |
- |
- |
Oryctolagus cuniculus | - |
- |
- |
Ovis aries | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Rhodospirillum rubrum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
20000 fold by DE-52, phenyl-Sepharose, hydroxylapatite, Affi-Gel 501 organo-mercurial agarose, Ultrogel AcA 54 | Rattus norvegicus |
200000fold by DE-52, phenyl-Sepharose, hydroxylapatite, Ultrogel AcA 54, Mono Q chromatography | Meleagris gallopavo |
partial | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Cavia porcellus | - |
brain | - |
Mus musculus | - |
brain | - |
Rattus norvegicus | - |
brain | - |
Bos taurus | - |
brain | - |
Oryctolagus cuniculus | - |
brain | - |
Ovis aries | - |
erythrocyte | - |
Meleagris gallopavo | - |
spleen | - |
Rattus norvegicus | - |
testis | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-ribose-arginine + H2O | - |
Meleagris gallopavo | ADP-ribose + arginine | - |
? | |
ADP-ribose-arginine + H2O | the existence of a ADP-ribosylation cycle was established in the bacterium Rhodospirillium rubrum where it regulates dinitrogenase reductase, a key enzyme in nitrogen fixation | Rhodospirillum rubrum | ADP-ribose + arginine | - |
? | |
alpha-subunit of stimulating GTP-binding protein ADP-ribosylated + H2O | - |
Rattus norvegicus | alpha-subunit of stimulating GTP-binding protein + ADP-ribose | - |
? | |
cholera toxin A1-subunit ADP-ribosylated + H2O | - |
Rattus norvegicus | cholera toxin A1-subunit + ADP-ribose | - |
? | |
nonmuscle actin ADP-ribosylated + H2O | - |
Rattus norvegicus | nonmuscle actin + ADP-ribose | - |
? |