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Literature summary for 3.2.2.19 extracted from
- ADP-ribosylarginine glycohydrolase catalyzing the release of ADP-ribose from the cholera toxin-modified alpha-subunits of GTP-binding proteins (1994), J. Biochem., 116, 1134-1138.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | as a GST-fusion protein | Rattus norvegicus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression as a glutathione S-transferase-linked fusion protein in Escherichia coli | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | inhibition with 1 mM ADP | Rattus norvegicus | |
| ADP-ribosylarginine methyl ester | - |
Rattus norvegicus | |
| ADPribose | inhibition with 1 mM ADP-ribose | Rattus norvegicus | |
| alphaNAD+ | inhibition with 1 mM alphaNAD+ | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
ADP-ribosylarginine methyl ester | as a GST-fusion protein | Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | as a GST-fusion protein | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| as a GST-fusion protein almost to homogeneity by affinity-column chromatography of glutathione-Sepharose | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-ribosylarginine methyl ester + H2O | - |
Rattus norvegicus | ADP-ribose + arginine methyl ester | - |
? | |
| alpha-subunit of stimulating GTP-binding protein ADP-ribosylated + H2O | the GDP-bound form of ADP-ribosylated stimulating GTP-binding protein-alpha is more rapidly hydrolyzed than the GTPgammaS-bound form | Rattus norvegicus | alpha-subunit of stimulating GTP-binding protein + ADP-ribose | - |
? | |
| cholera toxin A1-subunit ADP-ribosylated + H2O | - |
Rattus norvegicus | cholera toxin A1-subunit + ADP-ribose | - |
? | |
| nonmuscle actin ADP-ribosylated + H2O | - |
Rattus norvegicus | nonmuscle actin + ADP-ribose | - |
? | |
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Release 2023.1 (January 2023)
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