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Literature summary for 3.2.2.17 extracted from

  • Garvish, J.F.; Lloyd, R.S.
    The catalytic mechanism of a pyrimidine dimer-specific glycosylase(pdg)/abasic lyase, Chlorella virus-pdg (1999), J. Biol. Chem., 274, 9786-9794.
    View publication on PubMed
Search for more literature for 3.2.2.17

Cloned(Commentary)

Cloned (Comment) Organism
PCR-amplified from pUC19, subcloned into pET-11a Paramecium bursaria Chlorella virus 1

Organism

Organism UniProt Comment Textmining
Paramecium bursaria Chlorella virus 1
-
 Chlorella virus-pdg
-
Tequatrovirus T4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
mutants E23D and E23Q Paramecium bursaria Chlorella virus 1

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 cis-syn 49mer 0.19 nM, trans-syn II 49mer 6.0 nM, AP 49mer 0.15 nM Tequatrovirus T4
additional information
-
 cis-syn 49mer 0.09 nM, trans-syn II 49mer 1.5 nM, AP 49mer 0.17 nM Paramecium bursaria Chlorella virus 1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
 Tequatrovirus T4 thymine + ?
-
 ?
DNA + H2O enzyme mechanism glycosylase/AP lyase, cis-syn and trans-synII-isomers of cyclobutane pyrimidine dimers in double-stranded DNA Paramecium bursaria Chlorella virus 1 thymine + ?
-
 ?
oligonucleotides + H2O 49mers containing a site specific cis-syn, trans-syn I or trans-syn II thymine dimer, beta-elimination reaction Paramecium bursaria Chlorella virus 1 dCMP + dTMP
-
 ?

Synonyms

Synonyms Comment Organism
Chlorella virus pyrimidine dimer glycosylase
-
 Paramecium bursaria Chlorella virus 1
pyrimidine dimer-specific glycosylase/AP lyase
-
 Paramecium bursaria Chlorella virus 1