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Literature summary for 3.2.2.16 extracted from
- Substrate preference of 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase in Burkholderia thailandensis (2013), FEMS Microbiol. Lett., 339, 110-116.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Burkholderia thailandensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.019 | - |
S-methyl-5'-thioadenosine | at pH 7.5 and 25°C | Burkholderia thailandensis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
x * 34000, SDS-PAGE | Burkholderia thailandensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Burkholderia thailandensis | the enzyme also shows weak hydrolytic activity with S-adenosylhomocysteine | ? | - |
? | |
| S-methyl-5'-thioadenosine + H2O | Burkholderia thailandensis | preferred substrate | S-methyl-5-thio-D-ribose + adenine | - |
? | |
| S-methyl-5'-thioadenosine + H2O | Burkholderia thailandensis | almost completely preferred substrate | adenine + S-methyl-5-thio-D-ribose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia thailandensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His-bind resin column chromatography | Burkholderia thailandensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme also shows weak hydrolytic activity with S-adenosylhomocysteine | Burkholderia thailandensis | ? | - |
? | |
| S-methyl-5'-thioadenosine + H2O | preferred substrate | Burkholderia thailandensis | S-methyl-5-thio-D-ribose + adenine | - |
? | |
| S-methyl-5'-thioadenosine + H2O | almost completely preferred substrate | Burkholderia thailandensis | adenine + S-methyl-5-thio-D-ribose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34000, SDS-PAGE | Burkholderia thailandensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Burkholderia thailandensis |
| MTAN | - |
Burkholderia thailandensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 37 | - |
Burkholderia thailandensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 37 | - |
Burkholderia thailandensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.8 | - |
S-methyl-5'-thioadenosine | at pH 7.5 and 25°C | Burkholderia thailandensis | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | - |
Burkholderia thailandensis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 95 | - |
S-methyl-5'-thioadenosine | at pH 7.5 and 25°C | Burkholderia thailandensis | |
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