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Literature summary for 3.2.2.16 extracted from

  • Park, E.Y.; Choi, W.S.; Oh, S.I.; Kim, K.N.; Shin, J.S.; Song, H.K.
    Biochemical and structural characterization of 5-methylthioadenosine nucleosidases from Arabidopsis thaliana (2009), Biochem. Biophys. Res. Commun., 381, 619-624.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
isozymes AtMTAN1 and AtMTAN2, DNA and amino acid sequence determination and analysis, overexpression of isozyme AtMTAN2 in Escherichia coli Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment) Organism
isozymes AtMTAN1 and AtMTAN2, X-ray diffraction structure determination and analysis at 2.9 A resolution, molecular replacement, modelling Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-methyl-5'-thioadenosine + H2O Arabidopsis thaliana
-
S-methyl-5-thio-D-ribose + adenine
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
two isozymes AtMTAN1 and AtMTAN2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant isozyme AtMTAN2 from Escherichia coli to homogeneity Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificities of isozymes, isozyme AtMTAN2 is also active with S-adenosyl-L-homocysteine, EC 3.2.2.9, overview. Isozyme AtMTAN2 shows markedly broad substrate specificity for hydrolysis of S-adenosyl-L-homocysteine, while isozyme AtMTAN1 does not Arabidopsis thaliana ?
-
?
S-methyl-5'-thioadenosine + H2O
-
Arabidopsis thaliana S-methyl-5-thio-D-ribose + adenine
-
?

Subunits

Subunits Comment Organism
More comparison of secondary structures of isozymes AtMTAN1 and AtMTAN2, e.g. of a flexible loop, overview. Active site structures and structure modelling, overview Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
5'-methylthioadenosine nucleosidase
-
Arabidopsis thaliana
MTAN
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Arabidopsis thaliana