Cloned (Comment) | Organism |
---|---|
isozymes AtMTAN1 and AtMTAN2, DNA and amino acid sequence determination and analysis, overexpression of isozyme AtMTAN2 in Escherichia coli | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
isozymes AtMTAN1 and AtMTAN2, X-ray diffraction structure determination and analysis at 2.9 A resolution, molecular replacement, modelling | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-methyl-5'-thioadenosine + H2O | Arabidopsis thaliana | - |
S-methyl-5-thio-D-ribose + adenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
two isozymes AtMTAN1 and AtMTAN2 | - |
Purification (Comment) | Organism |
---|---|
recombinant isozyme AtMTAN2 from Escherichia coli to homogeneity | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificities of isozymes, isozyme AtMTAN2 is also active with S-adenosyl-L-homocysteine, EC 3.2.2.9, overview. Isozyme AtMTAN2 shows markedly broad substrate specificity for hydrolysis of S-adenosyl-L-homocysteine, while isozyme AtMTAN1 does not | Arabidopsis thaliana | ? | - |
? | |
S-methyl-5'-thioadenosine + H2O | - |
Arabidopsis thaliana | S-methyl-5-thio-D-ribose + adenine | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | comparison of secondary structures of isozymes AtMTAN1 and AtMTAN2, e.g. of a flexible loop, overview. Active site structures and structure modelling, overview | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
5'-methylthioadenosine nucleosidase | - |
Arabidopsis thaliana |
MTAN | - |
Arabidopsis thaliana |