Isolation and properties of a glycohydrolase specific for nicotinamide mononucleotide from Azotobacter vinelandii

Imai, T.; J. Biochem. 85, 887-899 (1979)

Data extracted from this reference:

Activating Compound

Commentary

Organism

Structure

ATP

less effective activator than GTP

Azotobacter vinelandii

dGMP

-

Azotobacter vinelandii

dGTP

-

Azotobacter vinelandii

GDP

-

Azotobacter vinelandii

GMP

-

Azotobacter vinelandii

GTP

most potent activator

Azotobacter vinelandii

guanosine 2'-monophosphate

-

Azotobacter vinelandii

guanosine 3'-monophosphate

-

Azotobacter vinelandii

guanosine 5'-tetraphosphate

most potent activator

Azotobacter vinelandii

Inhibitors

Commentary

Organism

Structure

Cd2+

1 mM

Azotobacter vinelandii

Cu2+

1 mM

Azotobacter vinelandii

dCMP

effective inhibition in presence of 1 mM GTP, noncompetitive inhibitor

Azotobacter vinelandii

dGMP

effective inhibition in presence of 1 mM GTP, noncompetitive inhibitor

Azotobacter vinelandii

EDTA

1 mM inhibits NMN hydrolysis by 30%

Azotobacter vinelandii

GMP

effective inhibition in presence of 1 mM GTP

Azotobacter vinelandii

N-ethylmaleimide

50% inhibition at 80 mM

Azotobacter vinelandii

p-chloromercuribenzoate

50% inhibition at 3 mM

Azotobacter vinelandii

Zn2+

1 mM

Azotobacter vinelandii

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

2

-

nicotinamide mononucleotide

in absence of GTP

Azotobacter vinelandii

4

-

nicotinamide mononucleotide

0.52 mM dGTP as effector

Azotobacter vinelandii

4.4

-

nicotinamide mononucleotide

0.5 mM guanosine 5'-tetraphosphate as effector

Azotobacter vinelandii

4.5

-

nicotinamide mononucleotide

0.5 mM/1 mM GTP

Azotobacter vinelandii

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

nicotinamide mononucleotide + H2O

Escherichia coli

conversion to nicotinamide as a precursor of NAD+ via nicotinic acid

?

-

-

-

nicotinamide mononucleotide + H2O

Azotobacter vinelandii

conversion to nicotinamide as a precursor of NAD+ via nicotinic acid

?

-

-

-

nicotinamide mononucleotide + H2O

Azotobacter vinelandii O

conversion to nicotinamide as a precursor of NAD+ via nicotinic acid

?

-

-

-

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

nicotinamide mononucleotide + H2O

-

171774

Escherichia coli

nicotinamide + ribose 5-phosphate

-

171774

Escherichia coli

-

nicotinamide mononucleotide + H2O

irreversible hydrolysis of the N-ribosidic linkage of NMN

171774

Azotobacter vinelandii

nicotinamide + ribose 5-phosphate

-

171774

Azotobacter vinelandii

ir

nicotinamide mononucleotide + H2O

irreversible hydrolysis of the N-ribosidic linkage of NMN

171774

Azotobacter vinelandii O

nicotinamide + ribose 5-phosphate

-

171774

Azotobacter vinelandii O

ir

nicotinamide mononucleotide + H2O

conversion to nicotinamide as a precursor of NAD+ via nicotinic acid

171774

Escherichia coli

?

-

-

-

-

nicotinamide mononucleotide + H2O

conversion to nicotinamide as a precursor of NAD+ via nicotinic acid

171774

Azotobacter vinelandii

?

-

-

-

-

nicotinamide mononucleotide + H2O

conversion to nicotinamide as a precursor of NAD+ via nicotinic acid

171774

Azotobacter vinelandii O

?

-

-

-

-

nicotinic acid mononucleotide + H2O

NaNM reaction slowly, 8% hydrolysis of the rate of NMN

171774

Azotobacter vinelandii

nicotinic acid + ribose 5-phosphate

-

171774

Azotobacter vinelandii

?

nicotinic acid mononucleotide + H2O

NaNM reaction slowly, 8% hydrolysis of the rate of NMN

171774

Azotobacter vinelandii O

nicotinic acid + ribose 5-phosphate

-

171774

Azotobacter vinelandii O

?

Ki Value [mM]

Ki Value maximum [mM]

Inhibitor

Commentary

Organism

Structure

3

-

p-chloromercuribenzoate

-

Azotobacter vinelandii

80

-

N-ethylmaleimide

-

Azotobacter vinelandii

Activating Compound

Commentary

Organism

Structure

ATP

less effective activator than GTP

Azotobacter vinelandii

dGMP

-

Azotobacter vinelandii

dGTP

-

Azotobacter vinelandii

GDP

-

Azotobacter vinelandii

GMP

-

Azotobacter vinelandii

GTP

most potent activator

Azotobacter vinelandii

guanosine 2'-monophosphate

-

Azotobacter vinelandii

guanosine 3'-monophosphate

-

Azotobacter vinelandii

guanosine 5'-tetraphosphate

most potent activator

Azotobacter vinelandii

Inhibitors

Commentary

Organism

Structure

Cd2+

1 mM

Azotobacter vinelandii

Cu2+

1 mM

Azotobacter vinelandii

dCMP

effective inhibition in presence of 1 mM GTP, noncompetitive inhibitor

Azotobacter vinelandii

dGMP

effective inhibition in presence of 1 mM GTP, noncompetitive inhibitor

Azotobacter vinelandii

EDTA

1 mM inhibits NMN hydrolysis by 30%

Azotobacter vinelandii

GMP

effective inhibition in presence of 1 mM GTP

Azotobacter vinelandii

N-ethylmaleimide

50% inhibition at 80 mM

Azotobacter vinelandii

p-chloromercuribenzoate

50% inhibition at 3 mM

Azotobacter vinelandii

Zn2+

1 mM

Azotobacter vinelandii

Ki Value [mM]

Ki Value maximum [mM]

Inhibitor

Commentary

Organism

Structure

3

-

p-chloromercuribenzoate

-

Azotobacter vinelandii

80

-

N-ethylmaleimide

-

Azotobacter vinelandii

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

2

-

nicotinamide mononucleotide

in absence of GTP

Azotobacter vinelandii

4

-

nicotinamide mononucleotide

0.52 mM dGTP as effector

Azotobacter vinelandii

4.4

-

nicotinamide mononucleotide

0.5 mM guanosine 5'-tetraphosphate as effector

Azotobacter vinelandii

4.5

-

nicotinamide mononucleotide

0.5 mM/1 mM GTP

Azotobacter vinelandii

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

nicotinamide mononucleotide + H2O

Escherichia coli

conversion to nicotinamide as a precursor of NAD+ via nicotinic acid

?

-

-

-

nicotinamide mononucleotide + H2O

Azotobacter vinelandii

conversion to nicotinamide as a precursor of NAD+ via nicotinic acid

?

-

-

-

nicotinamide mononucleotide + H2O

Azotobacter vinelandii O

conversion to nicotinamide as a precursor of NAD+ via nicotinic acid

?

-

-

-

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

nicotinamide mononucleotide + H2O

-

171774

Escherichia coli

nicotinamide + ribose 5-phosphate

-

171774

Escherichia coli

-

nicotinamide mononucleotide + H2O

irreversible hydrolysis of the N-ribosidic linkage of NMN

171774

Azotobacter vinelandii

nicotinamide + ribose 5-phosphate

-

171774

Azotobacter vinelandii

ir

nicotinamide mononucleotide + H2O

irreversible hydrolysis of the N-ribosidic linkage of NMN

171774

Azotobacter vinelandii O

nicotinamide + ribose 5-phosphate

-

171774

Azotobacter vinelandii O

ir

nicotinamide mononucleotide + H2O

conversion to nicotinamide as a precursor of NAD+ via nicotinic acid

171774

Escherichia coli

?

-

-

-

-

nicotinamide mononucleotide + H2O

conversion to nicotinamide as a precursor of NAD+ via nicotinic acid

171774

Azotobacter vinelandii

?

-

-

-

-

nicotinamide mononucleotide + H2O

conversion to nicotinamide as a precursor of NAD+ via nicotinic acid

171774

Azotobacter vinelandii O

?

-

-

-

-

nicotinic acid mononucleotide + H2O

NaNM reaction slowly, 8% hydrolysis of the rate of NMN

171774

Azotobacter vinelandii

nicotinic acid + ribose 5-phosphate

-

171774

Azotobacter vinelandii

?

nicotinic acid mononucleotide + H2O

NaNM reaction slowly, 8% hydrolysis of the rate of NMN

171774

Azotobacter vinelandii O

nicotinic acid + ribose 5-phosphate

-

171774

Azotobacter vinelandii O

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

732384

Ashihara

Pyridine metabolism in tea pla ...

Camellia sinensis

J. Plant Res.

125

781-791

2012

-

-

-

-

-

-

-

-

-

-

-

1

-

1

-

-

-

-

-

-

-

-

1

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

171776

Denicola-Seoane

Studies of NAD kinase and NMN: ...

Haemophilus influenzae, Haemophilus influenzae RD

J. Gen. Microbiol.

136

425-430

1990

-

-

-

-

-

-

-

-

-

-

-

2

-

7

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

171769

Imai

Properties of allosteric nicot ...

Azotobacter vinelandii

J. Biochem.

101

163-173

1987

3

-

-

-

-

-

4

1

-

-

3

1

-

2

-

-

1

-

-

-

-

-

2

1

-

-

-

-

-

-

-

-

-

-

-

-

3

-

-

-

-

-

-

-

4

-

1

-

-

3

1

-

-

-

1

-

-

-

-

2

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

171770

Imai

Isolation and characterization ...

Azotobacter vinelandii

J. Biochem.

101

153-161

1987

2

-

-

-

-

-

-

-

-

-

-

-

-

4

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

171771

Wagner

The pyridine-nucleotide cycle ...

Nicotiana tabacum

Planta

167

226-232

1986

-

-

-

-

-

-

-

1

-

-

-

1

-

1

-

-

1

-

-

1

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

1

-

-

-

1

-

1

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

171772

Wagner

Regulation in tobacco callus o ...

Nicotiana tabacum

Planta

168

408-413

1986

-

-

-

-

-

-

-

-

-

-

-

1

-

1

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

1

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

171773

Foster

Pyridine nucleotide cycle of S ...

Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2

J. Bacteriol.

145

1002-1009

1981

-

-

-

-

-

-

14

-

-

-

1

2

-

44

-

-

-

-

-

-

17

-

2

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

14

-

-

-

-

1

2

-

-

-

-

-

-

17

-

2

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

171774

Imai

Isolation and properties of a ...

Azotobacter vinelandii, Azotobacter vinelandii O, Escherichia coli

J. Biochem.

85

887-899

1979

9

-

-

-

-

1

9

4

-

-

2

3

-

4

-

-

1

-

-

-

3

2

8

-

-

1

-

-

-

1

-

-

-

2

-

-

9

-

-

-

-

-

1

-

9

2

4

-

-

2

3

-

-

-

1

-

-

3

2

8

-

1

-

-

-

1

-

-

-

-

-

-

-

-

-

171775

Andreoli

The pyridine nucleotide cycle: ...

Escherichia coli

Biochem. Biophys. Res. Commun.

49

264-269

1972

-

-

-

-

-

-

-

-

1

-

-

1

-

1

-

-

1

-

-

-

1

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

1

-

-

-

1

-

-

1

-

2

-

-

-

-

-

-

-

-

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-

-

-

-

-

-