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Literature summary for 3.2.2.1 extracted from

  • Singh, R.K.; Steyaert, J.; Versees, W.
    Structural and biochemical characterization of the nucleoside hydrolase from C. elegans reveals the role of two active site cysteine residues in catalysis (2017), Protein Sci., 26, 985-996 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Caenorhabditis elegans

Crystallization (Commentary)

Crystallization (Comment) Organism
to 1.65 A resolution. Both residues Cys 253 and Cys 42 are appropriately positioned to interact with the purine ring of the substrate Caenorhabditis elegans

Protein Variants

Protein Variants Comment Organism
C253A kcat/KM values for the hydrolysis of inosine, adenosine and guanosine are reduced by a factor of about 120fold compared to the wild-tpe enzyme Caenorhabditis elegans
C42A the kcat/KM values for the hydrolysis of inosine, adenosine, and guanosine are reduced by a factor of about 14-, 22-, and 10fold, respectively, compared to the wild-type enzyme Caenorhabditis elegans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00032
-
 4-nitrophenylriboside wild-type, pH 7.5, 25°C Caenorhabditis elegans
0.00064
-
 4-nitrophenylriboside mutant C42A, pH 7.5, 25°C Caenorhabditis elegans
0.009
-
 4-nitrophenylriboside mutant C253A, ApH 7.5, 25°C Caenorhabditis elegans
0.037
-
 7-methylguanosine wild-type, pH 7.5, 25°C Caenorhabditis elegans
0.051
-
 7-methylguanosine mutant C253A, ApH 7.5, 25°C Caenorhabditis elegans
0.053
-
 7-methylguanosine mutant C42A, pH 7.5, 25°C Caenorhabditis elegans
0.054
-
 guanosine mutant C42A, pH 7.5, 25°C Caenorhabditis elegans
0.068
-
 guanosine wild-type, pH 7.5, 25°C Caenorhabditis elegans
0.077
-
 adenosine wild-type, pH 7.5, 25°C Caenorhabditis elegans
0.094
-
 adenosine mutant C42A, pH 7.5, 25°C Caenorhabditis elegans
0.096
-
 adenosine mutant C253A, ApH 7.5, 25°C Caenorhabditis elegans
0.129
-
 Inosine wild-type, pH 7.5, 25°C Caenorhabditis elegans
0.215
-
 Inosine mutant C42A, pH 7.5, 25°C Caenorhabditis elegans
0.3
-
 guanosine mutant C253A, ApH 7.5, 25°C Caenorhabditis elegans
0.343
-
 Inosine mutant C253A, ApH 7.5, 25°C Caenorhabditis elegans

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans Q9XWN7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Caenorhabditis elegans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenylriboside + H2O
-
 Caenorhabditis elegans D-ribose + 4-nitrophenol
-
 ?
7-methylguanosine + H2O
-
 Caenorhabditis elegans D-ribose + 7-methylguanine
-
 ?
adenosine + H2O
-
 Caenorhabditis elegans D-ribose + adenine
-
 ?
guanosine + H2O
-
 Caenorhabditis elegans D-ribose + guanine
-
 ?
inosine + H2O
-
 Caenorhabditis elegans D-ribose + hypoxanthine
-
 ?

Synonyms

Synonyms Comment Organism
CELE_Y43F8C.13
-
 Caenorhabditis elegans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0042
-
 adenosine mutant C253A, ApH 7.5, 25°C Caenorhabditis elegans
0.0043
-
 Inosine mutant C253A, ApH 7.5, 25°C Caenorhabditis elegans
0.0051
-
 guanosine mutant C253A, ApH 7.5, 25°C Caenorhabditis elegans
0.011
-
 guanosine mutant C42A, pH 7.5, 25°C Caenorhabditis elegans
0.017
-
 4-nitrophenylriboside mutant C253A, ApH 7.5, 25°C Caenorhabditis elegans
0.023
-
 adenosine mutant C42A, pH 7.5, 25°C Caenorhabditis elegans
0.024
-
 Inosine mutant C42A, pH 7.5, 25°C Caenorhabditis elegans
0.026
-
 4-nitrophenylriboside mutant C42A, pH 7.5, 25°C Caenorhabditis elegans
0.039
-
 4-nitrophenylriboside wild-type, pH 7.5, 25°C Caenorhabditis elegans
0.143
-
 guanosine wild-type, pH 7.5, 25°C Caenorhabditis elegans
0.203
-
 Inosine wild-type, pH 7.5, 25°C Caenorhabditis elegans
0.418
-
 adenosine wild-type, pH 7.5, 25°C Caenorhabditis elegans
1.09
-
 7-methylguanosine wild-type, pH 7.5, 25°C Caenorhabditis elegans
1.98
-
 7-methylguanosine mutant C253A, ApH 7.5, 25°C Caenorhabditis elegans
2.4
-
 7-methylguanosine mutant C42A, pH 7.5, 25°C Caenorhabditis elegans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.013
-
 Inosine mutant C253A, ApH 7.5, 25°C Caenorhabditis elegans
0.017
-
 guanosine mutant C253A, ApH 7.5, 25°C Caenorhabditis elegans
0.044
-
 adenosine mutant C253A, ApH 7.5, 25°C Caenorhabditis elegans
0.112
-
 Inosine mutant C42A, pH 7.5, 25°C Caenorhabditis elegans
0.202
-
 guanosine mutant C42A, pH 7.5, 25°C Caenorhabditis elegans
0.245
-
 adenosine mutant C42A, pH 7.5, 25°C Caenorhabditis elegans
1.574
-
 Inosine wild-type, pH 7.5, 25°C Caenorhabditis elegans
1.89
-
 4-nitrophenylriboside mutant C253A, ApH 7.5, 25°C Caenorhabditis elegans
2.103
-
 guanosine wild-type, pH 7.5, 25°C Caenorhabditis elegans
5.429
-
 adenosine wild-type, pH 7.5, 25°C Caenorhabditis elegans
29.46
-
 7-methylguanosine wild-type, pH 7.5, 25°C Caenorhabditis elegans
38.82
-
 7-methylguanosine mutant C253A, ApH 7.5, 25°C Caenorhabditis elegans
40.63
-
 4-nitrophenylriboside mutant C42A, pH 7.5, 25°C Caenorhabditis elegans
45.28
-
 7-methylguanosine mutant C42A, pH 7.5, 25°C Caenorhabditis elegans
121.88
-
 4-nitrophenylriboside wild-type, pH 7.5, 25°C Caenorhabditis elegans