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Literature summary for 3.2.2.1 extracted from

  • Giannese, F.; Berg, M.; Van der Veken, P.; Castagna, V.; Tornaghi, P.; Augustyns, K.; Degano, M.
    Structures of purine nucleosidase from Trypanosoma brucei bound to isozyme-specific trypanocidals and a novel metalorganic inhibitor (2013), Acta Crystallogr. Sect. D, 69, 1553-1566.
    View publication on PubMed
Search for more literature for 3.2.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Trypanosoma brucei

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant detagged enzyme, in complex with iminoribitol-based competitive inhibitors UAMC-00363, immucillin-A and UAMC-00312, X-ray diffraction structure determination and analysis at 1.28-2.18 A resolution Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
Co2+ mixed-type inhibition Trypanosoma brucei
Cu2+ non-competitive inhibition Trypanosoma brucei
immucillin-A competitive inhibition Trypanosoma brucei
Mn2+ mixed-type inhibition Trypanosoma brucei
additional information high-affinity inhibition of the enzyme by iminoribitol-based compounds. Metallorganic complexes can compete for binding at the active site of nucleoside hydrolase enzymes, mimicking the positively charged transition state of the enzymatic reaction, different modes of iminoribitol ring binding at a conserved active site, overview. Divalent metal ions can act as noncompetitive enzyme inhibitors, stabilizing a nonproductive conformation of the catalytic loop Trypanosoma brucei
Ni2+ mixed-type inhibition Trypanosoma brucei
UAMC-00312 competitive inhibition Trypanosoma brucei
UAMC-00363 a trypanocidal compound, competitive inhibition Trypanosoma brucei
Zn2+ mixed-type inhibition Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 adenosine recombinant enzyme, pH 7.3, 37°C Trypanosoma brucei
0.051
-
 Inosine recombinant enzyme, pH 7.3, 37°C Trypanosoma brucei
0.075
-
 guanosine recombinant enzyme, pH 7.3, 37°C Trypanosoma brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
 subsp. brucei
-
Trypanosoma brucei TREU927
-
 subsp. brucei
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the tag is cleaved off by thrombin, followed by gel filtration Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine + H2O
-
 Trypanosoma brucei adenine + D-ribose
-
 ?
adenosine + H2O
-
 Trypanosoma brucei TREU927 adenine + D-ribose
-
 ?
guanosine + H2O
-
 Trypanosoma brucei guanine + D-ribose
-
 ?
guanosine + H2O
-
 Trypanosoma brucei TREU927 guanine + D-ribose
-
 ?
inosine + H2O
-
 Trypanosoma brucei hypoxanthine + D-ribose
-
 ?
inosine + H2O
-
 Trypanosoma brucei TREU927 hypoxanthine + D-ribose
-
 ?
additional information cytidine, uridine, and 4-nitrophenyl-riboside are poor substrates Trypanosoma brucei ?
-
 ?
additional information cytidine, uridine, and 4-nitrophenyl-riboside are poor substrates Trypanosoma brucei TREU927 ?
-
 ?

Synonyms

Synonyms Comment Organism
IAGNH
-
 Trypanosoma brucei
purine-specific nucleoside hydrolase
-
 Trypanosoma brucei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Trypanosoma brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17.3
-
 adenosine recombinant enzyme, pH 7.3, 37°C Trypanosoma brucei
37.3
-
 guanosine recombinant enzyme, pH 7.3, 37°C Trypanosoma brucei
61
-
 Inosine recombinant enzyme, pH 7.3, 37°C Trypanosoma brucei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
 assay at Trypanosoma brucei

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0009
-
 Cu2+ noncompetitive, recombinant enzyme, pH 7.3, 37°C Trypanosoma brucei
0.0028
-
 Zn2+ competitive, recombinant enzyme, pH 7.3, 37°C Trypanosoma brucei
0.0167
-
 Zn2+ noncompetitive, recombinant enzyme, pH 7.3, 37°C Trypanosoma brucei
0.028
-
 Ni2+ competitive, recombinant enzyme, pH 7.3, 37°C Trypanosoma brucei
0.16
-
 Co2+ competitive, recombinant enzyme, pH 7.3, 37°C Trypanosoma brucei
0.29
-
 Mn2+ competitive, recombinant enzyme, pH 7.3, 37°C Trypanosoma brucei
0.39
-
 Ni2+ noncompetitive, recombinant enzyme, pH 7.3, 37°C Trypanosoma brucei
1
-
 Mn2+ noncompetitive, recombinant enzyme, pH 7.3, 37°C Trypanosoma brucei
1
-
 Co2+ noncompetitive, recombinant enzyme, pH 7.3, 37°C Trypanosoma brucei

General Information

General Information Comment Organism
additional information molecular mechanism underlying the conformational change necessary for enzymatic catalysis, overview Trypanosoma brucei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
499
-
 guanosine recombinant enzyme, pH 7.3, 37°C Trypanosoma brucei
1090
-
 adenosine recombinant enzyme, pH 7.3, 37°C Trypanosoma brucei
1190
-
 Inosine recombinant enzyme, pH 7.3, 37°C Trypanosoma brucei