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Literature summary for 3.2.2.1 extracted from

  • Minici, C.; Cacciapuoti, G.; De Leo, E.; Porcelli, M.; Degano, M.
    New determinants in the catalytic mechanism of nucleoside hydrolases from the structures of two isozymes from Sulfolobus solfataricus (2012), Biochemistry, 51, 4590-4599.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure is determined at 1.8 A resolution. It is crystallized using the hanging drop vapor diffusion method, mixing 0.001 ml of protein and 0.001 ml of aprecipitant solution consisting of 100 mM Hepes (pH 7.5), 5% PEG 3350, 5 mM CaCl2, 5 mM CdCl2, and 5 mM MgCl2 Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
H79A kcat/Km for inosine is 13fold lower than wild-type value Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
Inosine mutant enzyme H79A, pH and temperature not specified in the publication Saccharolobus solfataricus
0.34
-
Inosine wild-type enzyme, pH and temperature not specified in the publication Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the active site contains the trademark Ca2+ ion of nucleodide hydrolase enzymes, octacoordinated by residues Asp9, Asp14, Ile121, and Asp238, two solvent molecules, and one glycerol molecule Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97WH6
-
-
Saccharolobus solfataricus P2 Q97WH6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inosine + H2O
-
Saccharolobus solfataricus hypoxanthine + D-ribose
-
?
inosine + H2O
-
Saccharolobus solfataricus P2 hypoxanthine + D-ribose
-
?

Synonyms

Synonyms Comment Organism
purine-preferring nucleoside hydrolase
-
Saccharolobus solfataricus
purine-specific inosine-adenosine-guanosine nucleoside hydrolase
-
Saccharolobus solfataricus
SsIAG-NH
-
Saccharolobus solfataricus
SSO2243 locus name Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.85
-
Inosine mutant enzyme H79A, pH and temperature not specified in the publication Saccharolobus solfataricus
11.6
-
Inosine wild-type enzyme, pH and temperature not specified in the publication Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6
-
Inosine mutant enzyme H79A, pH and temperature not specified in the publication Saccharolobus solfataricus
34.1
-
Inosine wild-type enzyme, pH and temperature not specified in the publication Saccharolobus solfataricus