Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli | Saccharolobus solfataricus |
Crystallization (Comment) | Organism |
---|---|
crystal structure is determined at 1.8 A resolution. It is crystallized using the hanging drop vapor diffusion method, mixing 0.001 ml of protein and 0.001 ml of aprecipitant solution consisting of 100 mM Hepes (pH 7.5), 5% PEG 3350, 5 mM CaCl2, 5 mM CdCl2, and 5 mM MgCl2 | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
H79A | kcat/Km for inosine is 13fold lower than wild-type value | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
Inosine | mutant enzyme H79A, pH and temperature not specified in the publication | Saccharolobus solfataricus | |
0.34 | - |
Inosine | wild-type enzyme, pH and temperature not specified in the publication | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the active site contains the trademark Ca2+ ion of nucleodide hydrolase enzymes, octacoordinated by residues Asp9, Asp14, Ile121, and Asp238, two solvent molecules, and one glycerol molecule | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97WH6 | - |
- |
Saccharolobus solfataricus P2 | Q97WH6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inosine + H2O | - |
Saccharolobus solfataricus | hypoxanthine + D-ribose | - |
? | |
inosine + H2O | - |
Saccharolobus solfataricus P2 | hypoxanthine + D-ribose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
purine-preferring nucleoside hydrolase | - |
Saccharolobus solfataricus |
purine-specific inosine-adenosine-guanosine nucleoside hydrolase | - |
Saccharolobus solfataricus |
SsIAG-NH | - |
Saccharolobus solfataricus |
SSO2243 | locus name | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.85 | - |
Inosine | mutant enzyme H79A, pH and temperature not specified in the publication | Saccharolobus solfataricus | |
11.6 | - |
Inosine | wild-type enzyme, pH and temperature not specified in the publication | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
Inosine | mutant enzyme H79A, pH and temperature not specified in the publication | Saccharolobus solfataricus | |
34.1 | - |
Inosine | wild-type enzyme, pH and temperature not specified in the publication | Saccharolobus solfataricus |