Literature summary for 3.2.2.1 extracted from

Takagi, Y.; Horecker, B.L.
Purification and properties of a bacterial riboside riboside hydrolase (1956), J. Biol. Chem., 225, 77-86.

Search for more literature for 3.2.2.1

General Stability

General Stability	Organism
much activity is lost on lyophilization or dialysis even against the alkaline buffers	Lactobacillus delbrueckii

Organism

Organism	UniProt	Comment	Textmining
Lactobacillus delbrueckii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Lactobacillus delbrueckii

Storage Stability

Storage Stability	Organism
in frozen state, stable for more than 6 months	Lactobacillus delbrueckii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,6-diamino-9-beta-D-ribofuranosylpurine + H2O	76% of the activity with inosine	Lactobacillus delbrueckii	2,6-diaminopurine + D-ribose	-	ir
2,8-diamino-9-beta-D-ribofuranosyladenine + H2O	38% of the activity with inosine	Lactobacillus delbrueckii	2,8-diaminoadenine + D-ribose	-	ir
2-hydroxy-9-beta-D-ribofuranosyladenine + H2O	10% of the activity with inosine	Lactobacillus delbrueckii	2-hydroxyadenine + D-ribose	-	ir
5,6-dimethyl-1-beta-D-ribofuranosylbenzimidazole + H2O	200% of the activity with inosine	Lactobacillus delbrueckii	5,6-dimethylbenzimidazole + D-ribose	-	ir
6-dimethylamino-9-beta-D-ribofuranosylbenzimidazole + H2O	30% of the activity with inosine	Lactobacillus delbrueckii	6-dimethylaminobenzimidazole + D-ribose	-	ir
9-beta-D-ribofuranosylpurine + H2O	47% of the activity with inosine	Lactobacillus delbrueckii	purine + D-ribose	-	ir
adenosine + H2O	103% of the activity with inosine	Lactobacillus delbrueckii	D-ribose + adenine	-	ir
cytidine + H2O	54% of the activity with inosine	Lactobacillus delbrueckii	D-ribose + cytosine	-	ir
guanosine + H2O	90% of the activity with inosine	Lactobacillus delbrueckii	D-ribose + guanine	-	ir
imidazoleacetic acid riboside + H2O	5% of the activity with inosine	Lactobacillus delbrueckii	imidazoleacetate + D-ribose	-	ir
inosine + H2O	-	Lactobacillus delbrueckii	hypoxanthine + D-ribose	-	ir
nicotinamide riboside + H2O	150% of the activity with inosine	Lactobacillus delbrueckii	nicotinamide + D-ribose	-	ir
uridine + H2O	-	Lactobacillus delbrueckii	D-ribose + uracil	-	ir

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	hydrolysis of inosine or uridine	Lactobacillus delbrueckii

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
6.2	-	unstable	Lactobacillus delbrueckii
7.5	-	stable	Lactobacillus delbrueckii
8.6	-	stable	Lactobacillus delbrueckii

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Release 2023.1 (January 2023)