Any feedback?
Literature summary for 3.2.1.B35 extracted from
- Exchange of active site residues alters substrate specificity in extremely thermostable beta-glycosidase from Thermococcus kodakarensis KOD1 (2015), Enzyme Microb. Technol., 77, 14-20.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermococcus kodakarensis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D206N | mutation alters the catalytic turn-over rate for glucosidase and mannosidase activities with fucosidase activity remain unchanged | Thermococcus kodakarensis |
| D206Q | catalytically inactive mutant enzyme. The extended side chain of D206Q is predicted to affect the substrate binding during catalysis | Thermococcus kodakarensis |
| E207S | catalytically inactive mutant enzyme | Thermococcus kodakarensis |
| E399S | catalytically inactive mutant enzyme | Thermococcus kodakarensis |
| Q77R | catalytically inactive mutant enzyme. Q77R might have made some changes in three dimensional structure due to its electrostatic effect and lost its catalytic activity | Thermococcus kodakarensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
4-nitrophenyl beta-D-mannopyranoside | 80°C, pH not specified in the publication, mutant enzyme D206N | Thermococcus kodakarensis |  |
| 0.94 | - |
4-nitrophenyl beta-D-fucopyranoside | 80°C, pH not specified in the publication, wild-type enzyme | Thermococcus kodakarensis | |
| 1.08 | - |
4-nitrophenyl beta-D-mannopyranoside | 80°C, pH not specified in the publication, wild-type enzyme | Thermococcus kodakarensis | |
| 1.29 | - |
4-nitrophenyl beta-D-fucopyranoside | 80°C, pH not specified in the publication, mutant enzyme D206N | Thermococcus kodakarensis | |
| 4.6 | - |
4-nitrophenyl beta-D-glucopyranoside | 80°C, pH not specified in the publication, wild-type enzyme | Thermococcus kodakarensis | |
| 9.23 | - |
4-nitrophenyl beta-D-glucopyranoside | 80°C, pH not specified in the publication, mutant enzyme D206N | Thermococcus kodakarensis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus kodakarensis | Q9YGB8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermococcus kodakarensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Thermococcus kodakarensis | 4-nitrophenol + beta-D-fucopyranose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermococcus kodakarensis | 4-nitrophenol + D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-mannopyranoside + H2O | - |
Thermococcus kodakarensis | 4-nitrophenol + beta-D-mannopyranose | - |
? | |
| additional information | the enzyme shows beta-glucosidase, beta-mannosidase, beta-fucosidase and beta-galactosidase activities | Thermococcus kodakarensis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | wild-type and mutant enzymes (Q77R, D206N, D206Q, E207S and E399S) | Thermococcus kodakarensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | 100 | - |
Thermococcus kodakarensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 100 | 60°C: about 40% of maximal activity, 95-100°C: optimum | Thermococcus kodakarensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 20.69 | - |
4-nitrophenyl beta-D-mannopyranoside | 80°C, pH not specified in the publication, mutant enzyme D206N | Thermococcus kodakarensis | |
| 50.99 | - |
4-nitrophenyl beta-D-mannopyranoside | 80°C, pH not specified in the publication, wild-type enzyme | Thermococcus kodakarensis | |
| 227.6 | - |
4-nitrophenyl beta-D-glucopyranoside | 80°C, pH not specified in the publication, wild-type enzyme | Thermococcus kodakarensis | |
| 322.3 | - |
4-nitrophenyl beta-D-fucopyranoside | 80°C, pH not specified in the publication, wild-type enzyme | Thermococcus kodakarensis | |
| 323.4 | - |
4-nitrophenyl beta-D-fucopyranoside | 80°C, pH not specified in the publication, mutant enzyme D206N | Thermococcus kodakarensis | |
| 340.6 | - |
4-nitrophenyl beta-D-glucopyranoside | 80°C, pH not specified in the publication, mutant enzyme D206N | Thermococcus kodakarensis | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 36.9 | - |
4-nitrophenyl beta-D-glucopyranoside | 80°C, pH not specified in the publication, mutant enzyme D206N | Thermococcus kodakarensis | |
| 47.2 | - |
4-nitrophenyl beta-D-mannopyranoside | 80°C, pH not specified in the publication, wild-type enzyme | Thermococcus kodakarensis | |
| 49.48 | - |
4-nitrophenyl beta-D-glucopyranoside | 80°C, pH not specified in the publication, wild-type enzyme | Thermococcus kodakarensis | |
| 51.7 | - |
4-nitrophenyl beta-D-mannopyranoside | 80°C, pH not specified in the publication, mutant enzyme D206N | Thermococcus kodakarensis | |
| 250.7 | - |
4-nitrophenyl beta-D-fucopyranoside | 80°C, pH not specified in the publication, mutant enzyme D206N | Thermococcus kodakarensis | |
| 342.8 | - |
4-nitrophenyl beta-D-fucopyranoside | 80°C, pH not specified in the publication, wild-type enzyme | Thermococcus kodakarensis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
