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Literature summary for 3.2.1.B31 extracted from
- Purification and characterization of flavonol 3-glucosidase, and its activity during ripening in tartary buckwheat seeds (2002), Plant Sci., 163, 417-423.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.115 | - |
rutin | pH 5.0, 37°C, isoenzyme f3gI | Fagopyrum tataricum |  |
| 0.125 | - |
rutin | pH 5.0, 37°C, isoenzyme f3gII | Fagopyrum tataricum | |
| 1.05 | - |
isoquercitrin | pH 5.0, 37°C, isoenzyme f3gI | Fagopyrum tataricum | |
| 1.15 | - |
isoquercitrin | pH 5.0, 37°C, isoenzyme f3gII | Fagopyrum tataricum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57400 | - |
x * 57400, isoenzyme f3gII, SDS-PAGE | Fagopyrum tataricum |
| 58200 | - |
x * 58200, isoenzyme f3gI, SDS-PAGE | Fagopyrum tataricum |
| 89000 | - |
isoenzyme f3gI and isoenzyme f4gII, gel filtration | Fagopyrum tataricum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fagopyrum tataricum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Fagopyrum tataricum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | testa | Fagopyrum tataricum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 135.7 | - |
pH 5.0, 37°C | Fagopyrum tataricum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isoquercitrin + H2O | i.e. quercetin 3-O-beta-D-glucoside, Vmax value for isoquercitrin is one-tenth of that for rutin | Fagopyrum tataricum | quercetin + beta-D-glucose | - |
? | |
| rutin + H2O | i.e. quercetin 3-O-beta-D-rutenoside | Fagopyrum tataricum | quercetin + rutinose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 57400, isoenzyme f3gII, SDS-PAGE | Fagopyrum tataricum |
| ? | x * 58200, isoenzyme f3gI, SDS-PAGE | Fagopyrum tataricum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| flavonol 3-glucosidase | - |
Fagopyrum tataricum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Fagopyrum tataricum |
| 40 | - |
- |
Fagopyrum tataricum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Fagopyrum tataricum |
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Release 2023.1 (January 2023)
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