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Literature summary for 3.2.1.B31 extracted from
- Purification and characterization of a flavonol 3-O-beta-heterodisaccharidase from the dried herb of Fagopyrum esculentum Moench (2003), Phytochemistry, 64, 411-418.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ethanol | completely inactivated by ethanolH2O of above 1:2 (v/v) | Fagopyrum esculentum |  |
| methanol | methanol-H2O at 1:2 (v/v) leads to a loss of activity of about only 20%. The enzyme is completely inactivated by methanol-H2O at above 1:1 (v/v) | Fagopyrum esculentum | |
| quercitrin | weak | Fagopyrum esculentum | |
| troxerutin | weak | Fagopyrum esculentum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.375 | - |
isorhamnetin 3-O-rutinoside | 30°C, pH not specified in the publication | Fagopyrum esculentum | |
| 0.561 | - |
rutin | 30°C, pH not specified in the publication | Fagopyrum esculentum | |
| 0.95 | - |
kaempferol 3-O-rutinoside | 30°C, pH not specified in the publication | Fagopyrum esculentum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 74500 | - |
1 * 74500, SDS-PAGE under reducing conditions | Fagopyrum esculentum |
| 80000 | - |
gel filtration | Fagopyrum esculentum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fagopyrum esculentum | - |
- |
- |
| Fagopyrum esculentum Moench | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 23% carbohydrate content | Fagopyrum esculentum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Fagopyrum esculentum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isorhamnetin 3-O-rutinoside + H2O | specific activity 3% compared to rutin (quercetin 3-O-beta-rutenoside) | Fagopyrum esculentum | isorhamnetin + rutin | - |
? | |
| isorhamnetin 3-O-rutinoside + H2O | specific activity 3% compared to rutin (quercetin 3-O-beta-rutenoside) | Fagopyrum esculentum Moench | isorhamnetin + rutin | - |
? | |
| kaempferol 3-O-rutinoside + H2O | specific activity is 134% compared to rutin | Fagopyrum esculentum | kaempferol + rutinose | - |
? | |
| kaempferol 3-O-rutinoside + H2O | specific activity is 134% compared to rutin | Fagopyrum esculentum Moench | kaempferol + rutinose | - |
? | |
| additional information | the enzyme also shows glucosidase activity with kaempferol 3-O-sophoroside-7-O-glucoside, kaempferol 3-O-glucoside and kaempferol 3-O-glucoside-7-O-rhamnoside | Fagopyrum esculentum | ? | - |
? | |
| additional information | the enzyme also shows glucosidase activity with kaempferol 3-O-sophoroside-7-O-glucoside, kaempferol 3-O-glucoside and kaempferol 3-O-glucoside-7-O-rhamnoside | Fagopyrum esculentum Moench | ? | - |
? | |
| rutin + H2O | i.e. quercetin 3-O-beta-rutenoside. Rutinose is the only sugar released, glucose or rhamnose is not detected. Comparable kcat/Km values for rutin and kaempferol 3-O-rutinoside | Fagopyrum esculentum | quercetin + rutinose | - |
? | |
| rutin + H2O | i.e. quercetin 3-O-beta-rutenoside. Rutinose is the only sugar released, glucose or rhamnose is not detected. Comparable kcat/Km values for rutin and kaempferol 3-O-rutinoside | Fagopyrum esculentum Moench | quercetin + rutinose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 74500, SDS-PAGE under reducing conditions | Fagopyrum esculentum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FHG I | - |
Fagopyrum esculentum |
| flavonol-3-O-heterodisaccharide glucohydrolase | - |
Fagopyrum esculentum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Fagopyrum esculentum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 70 | 4°C: about 90% of maximal activity, 70°C: about 25% of maximal activity | Fagopyrum esculentum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 50 | stable | Fagopyrum esculentum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
- |
Fagopyrum esculentum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.9 | 7 | pH 3.9: about 50% of maximal activity, pH 7.0: about 50% of maximal activity | Fagopyrum esculentum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Fagopyrum esculentum | isoelectric focusing | - |
5.7 |
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