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Literature summary for 3.2.1.B31 extracted from
- A beta-rutinosidase from Penicillium rugulosum IFO 7242 that is a peculiar flavonoid glycosidase (2000), Biosci. Biotechnol. Biochem., 64, 1317-1379.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
x * 65000, SDS-PAGE | Talaromyces rugulosus |
| 245000 | - |
gel filtration | Talaromyces rugulosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Talaromyces rugulosus | - |
- |
- |
| Talaromyces rugulosus IFO 7242 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 596 | - |
pH and temperature not specified in the publication | Talaromyces rugulosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isoquercitrin + H2O | i.e. quercetin 3-O-beta-D-glucoside, hydrolyzed at 98.7% compared to rutin | Talaromyces rugulosus | quercetin + beta-D-glucose | - |
? |  |
| isoquercitrin + H2O | i.e. quercetin 3-O-beta-D-glucoside, hydrolyzed at 98.7% compared to rutin | Talaromyces rugulosus IFO 7242 | quercetin + beta-D-glucose | - |
? | |
| additional information | no activity with: quercitrin (i.e. quercetin 3-O-alpha-rhamnoside), hesperidin (i.e. hersperetin 7-O-beta-rutenoside), naringin (naringenin 7-O-beta-neohesperidoside), 4-nitrophenyl-alpha-rhamnoside, 4-nitrophenyl-beta-glucoside, methyl beta-glucoside, cellobiose | Talaromyces rugulosus | ? | - |
? | |
| additional information | no activity with: quercitrin (i.e. quercetin 3-O-alpha-rhamnoside), hesperidin (i.e. hersperetin 7-O-beta-rutenoside), naringin (naringenin 7-O-beta-neohesperidoside), 4-nitrophenyl-alpha-rhamnoside, 4-nitrophenyl-beta-glucoside, methyl beta-glucoside, cellobiose | Talaromyces rugulosus IFO 7242 | ? | - |
? | |
| rutin + H2O | i.e. quercetin 3-O-beta-rutenoside | Talaromyces rugulosus | quercetin + rutinose | - |
? | |
| rutin + H2O | i.e. quercetin 3-O-beta-rutenoside | Talaromyces rugulosus IFO 7242 | quercetin + rutinose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65000, SDS-PAGE | Talaromyces rugulosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-rutenosidase | - |
Talaromyces rugulosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Talaromyces rugulosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable below | Talaromyces rugulosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2.2 | - |
- |
Talaromyces rugulosus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 1.5 | 3 | pH 1.5: about 50% of maximal activity (KCl-HCl buffer), pH 3.0: about 80% of maximal activity (glycine-HCl buffer) | Talaromyces rugulosus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 11 | stable | Talaromyces rugulosus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Talaromyces rugulosus | isoelectric focusing | - |
5 |
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Release 2023.1 (January 2023)
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