BRENDA - Enzyme Database show
show all sequences of 3.2.1.B28

Hydrolysis of flavanone glycosides by beta-glucosidase from Pyrococcus furiosus and its application to the production of flavanone aglycones from citrus extracts.

Shin, K.C.; Nam, H.K.; Oh, D.K.; J. Agric. Food Chem. 61, 11532-11540 (2013)

Data extracted from this reference:

Application
Application
Commentary
Organism
additional information
the enzyme converts flavanone glycoside to flavanone aglycone via a one-step reaction. It may be useful in the production of the flavanone aglycones naringenin and hesperetin from flavanone glycosides in citrus extracts
Pyrococcus furiosus
Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Pyrococcus furiosus
Inhibitors
Inhibitors
Commentary
Organism
Structure
1-methyl-1-butanol
10% (v/v), about% inhibition
Pyrococcus furiosus
2-propanol
10% (v/v), about% inhibition
Pyrococcus furiosus
acetone
10% (v/v), about% inhibition
Pyrococcus furiosus
DMSO
10% (v/v), about% inhibition
Pyrococcus furiosus
ethanol
10% (v/v), about 50% inhibition
Pyrococcus furiosus
formate
10% (v/v), about% inhibition
Pyrococcus furiosus
Isopropanol
10% (v/v), about% inhibition
Pyrococcus furiosus
methanol
10% (v/v), about% inhibition
Pyrococcus furiosus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.6
-
hesperidin
pH 5.5, 95°C
Pyrococcus furiosus
1.7
-
naringin
pH 5.5, 95°C
Pyrococcus furiosus
3.7
-
narirutin
pH 5.5, 95°C
Pyrococcus furiosus
4.6
-
neohesperidin
pH 5.5, 95°C
Pyrococcus furiosus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55488
-
x * 55488, calculated from sequence
Pyrococcus furiosus
56000
-
x * 56000, SDS-PAGE
Pyrococcus furiosus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pyrococcus furiosus
Q51723
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.011
-
substrate: rutin, pH 5.5, 95°C
Pyrococcus furiosus
0.081
-
substrate: neoponcirin, pH 5.5, 95°C
Pyrococcus furiosus
0.085
-
substrate: diosmin, pH 5.5, 95°C
Pyrococcus furiosus
0.091
-
substrate: poncirin, pH 5.5, 95°C
Pyrococcus furiosus
0.102
-
substrate naringin, pH 5.5, 95°C
Pyrococcus furiosus
0.103
-
substrate: neohesperidin, pH 5.5, 95°C
Pyrococcus furiosus
0.153
-
substrate: narirutin, pH 5.5, 95°C
Pyrococcus furiosus
0.172
-
substrate: hesperidin, pH 5.5, 95°C
Pyrococcus furiosus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
diosmin + H2O
-
725181
Pyrococcus furiosus
diosmetin + rutinose
-
-
-
?
hesperidin + H2O
-
725181
Pyrococcus furiosus
hesperetin + rutinose
-
-
-
?
naringin + H2O
-
725181
Pyrococcus furiosus
naringenin + rutinose
-
-
-
?
narirutin + H2O
-
725181
Pyrococcus furiosus
naringenin + neohesperidose
-
-
-
?
neohesperidin + H2O
-
725181
Pyrococcus furiosus
hesperetin + neohesperidose
-
-
-
?
neoponcirin + H2O
-
725181
Pyrococcus furiosus
isosakuranetin + rutinose
-
-
-
?
poncirin + H2O
-
725181
Pyrococcus furiosus
isosakuranetin + neohesperidose
-
-
-
?
rutin + H2O
-
725181
Pyrococcus furiosus
quercetin + rutinose
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 55488, calculated from sequence; x * 56000, SDS-PAGE
Pyrococcus furiosus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
95
-
-
Pyrococcus furiosus
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
75
95
the activity increases from 75°C to 95°C, activity at 75°C is about 40% of the activity at 95°C, substrate: hesperidin
Pyrococcus furiosus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
75
-
half-life: 637 h
Pyrococcus furiosus
80
-
half-life: 392 h
Pyrococcus furiosus
85
-
half-life: 235 h
Pyrococcus furiosus
90
-
half-life: 138 h
Pyrococcus furiosus
95
-
half-life: 88 h
Pyrococcus furiosus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
27.7
-
naringin
pH 5.5, 95°C
Pyrococcus furiosus
68.4
-
hesperidin
pH 5.5, 95°C
Pyrococcus furiosus
74.1
-
narirutin
pH 5.5, 95°C
Pyrococcus furiosus
84.8
-
neohesperidin
pH 5.5, 95°C
Pyrococcus furiosus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
substrate: hesperidin
Pyrococcus furiosus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.5
6.5
pH 4.5: about 50% of maximal activity, pH 6.5: about 60% of maximal activity, substrate: hesperidin
Pyrococcus furiosus
Application (protein specific)
Application
Commentary
Organism
additional information
the enzyme converts flavanone glycoside to flavanone aglycone via a one-step reaction. It may be useful in the production of the flavanone aglycones naringenin and hesperetin from flavanone glycosides in citrus extracts
Pyrococcus furiosus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Pyrococcus furiosus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1-methyl-1-butanol
10% (v/v), about% inhibition
Pyrococcus furiosus
2-propanol
10% (v/v), about% inhibition
Pyrococcus furiosus
acetone
10% (v/v), about% inhibition
Pyrococcus furiosus
DMSO
10% (v/v), about% inhibition
Pyrococcus furiosus
ethanol
10% (v/v), about 50% inhibition
Pyrococcus furiosus
formate
10% (v/v), about% inhibition
Pyrococcus furiosus
Isopropanol
10% (v/v), about% inhibition
Pyrococcus furiosus
methanol
10% (v/v), about% inhibition
Pyrococcus furiosus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.6
-
hesperidin
pH 5.5, 95°C
Pyrococcus furiosus
1.7
-
naringin
pH 5.5, 95°C
Pyrococcus furiosus
3.7
-
narirutin
pH 5.5, 95°C
Pyrococcus furiosus
4.6
-
neohesperidin
pH 5.5, 95°C
Pyrococcus furiosus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55488
-
x * 55488, calculated from sequence
Pyrococcus furiosus
56000
-
x * 56000, SDS-PAGE
Pyrococcus furiosus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.011
-
substrate: rutin, pH 5.5, 95°C
Pyrococcus furiosus
0.081
-
substrate: neoponcirin, pH 5.5, 95°C
Pyrococcus furiosus
0.085
-
substrate: diosmin, pH 5.5, 95°C
Pyrococcus furiosus
0.091
-
substrate: poncirin, pH 5.5, 95°C
Pyrococcus furiosus
0.102
-
substrate naringin, pH 5.5, 95°C
Pyrococcus furiosus
0.103
-
substrate: neohesperidin, pH 5.5, 95°C
Pyrococcus furiosus
0.153
-
substrate: narirutin, pH 5.5, 95°C
Pyrococcus furiosus
0.172
-
substrate: hesperidin, pH 5.5, 95°C
Pyrococcus furiosus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
diosmin + H2O
-
725181
Pyrococcus furiosus
diosmetin + rutinose
-
-
-
?
hesperidin + H2O
-
725181
Pyrococcus furiosus
hesperetin + rutinose
-
-
-
?
naringin + H2O
-
725181
Pyrococcus furiosus
naringenin + rutinose
-
-
-
?
narirutin + H2O
-
725181
Pyrococcus furiosus
naringenin + neohesperidose
-
-
-
?
neohesperidin + H2O
-
725181
Pyrococcus furiosus
hesperetin + neohesperidose
-
-
-
?
neoponcirin + H2O
-
725181
Pyrococcus furiosus
isosakuranetin + rutinose
-
-
-
?
poncirin + H2O
-
725181
Pyrococcus furiosus
isosakuranetin + neohesperidose
-
-
-
?
rutin + H2O
-
725181
Pyrococcus furiosus
quercetin + rutinose
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 55488, calculated from sequence; x * 56000, SDS-PAGE
Pyrococcus furiosus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
95
-
-
Pyrococcus furiosus
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
75
95
the activity increases from 75°C to 95°C, activity at 75°C is about 40% of the activity at 95°C, substrate: hesperidin
Pyrococcus furiosus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
75
-
half-life: 637 h
Pyrococcus furiosus
80
-
half-life: 392 h
Pyrococcus furiosus
85
-
half-life: 235 h
Pyrococcus furiosus
90
-
half-life: 138 h
Pyrococcus furiosus
95
-
half-life: 88 h
Pyrococcus furiosus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
27.7
-
naringin
pH 5.5, 95°C
Pyrococcus furiosus
68.4
-
hesperidin
pH 5.5, 95°C
Pyrococcus furiosus
74.1
-
narirutin
pH 5.5, 95°C
Pyrococcus furiosus
84.8
-
neohesperidin
pH 5.5, 95°C
Pyrococcus furiosus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
substrate: hesperidin
Pyrococcus furiosus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.5
6.5
pH 4.5: about 50% of maximal activity, pH 6.5: about 60% of maximal activity, substrate: hesperidin
Pyrococcus furiosus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
16.5
-
naringin
pH 5.5, 95°C
Pyrococcus furiosus
18.5
-
neohesperidin
pH 5.5, 95°C
Pyrococcus furiosus
19.8
-
narirutin
pH 5.5, 95°C
Pyrococcus furiosus
42.1
-
hesperidin
pH 5.5, 95°C
Pyrococcus furiosus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
16.5
-
naringin
pH 5.5, 95°C
Pyrococcus furiosus
18.5
-
neohesperidin
pH 5.5, 95°C
Pyrococcus furiosus
19.8
-
narirutin
pH 5.5, 95°C
Pyrococcus furiosus
42.1
-
hesperidin
pH 5.5, 95°C
Pyrococcus furiosus
Other publictions for EC 3.2.1.B28
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746232
Choi
An L213A variant of beta-glyc ...
Pyrococcus furiosus
PLoS ONE
13
e0191018
2018
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2
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736853
Zeuner
Thermostable beta-galactosidas ...
Pyrococcus furiosus
N. Biotechnol.
33
355-60
2016
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1
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1
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1
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3
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3
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726640
Nakabayashi
Monomer structure of a hyperth ...
Pyrococcus furiosus
Acta Crystallogr. Sect. F
70
854-859
2014
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1
1
1
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1
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3
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735942
Watanabe
-
Encapsulation of hyperthermoph ...
Pyrococcus furiosus
Chem. Lett.
43
1272-1274
2014
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1
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723717
Park
Mutation studies in the active ...
Pyrococcus furiosus
Protein Pept. Lett.
20
107-114
2013
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-
1
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2
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-
10
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1
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1
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1
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5
1
1
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9
1
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1
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2
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10
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1
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1
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5
1
1
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9
1
-
-
-
-
-
-
-
10
10
725181
Shin
Hydrolysis of flavanone glycos ...
Pyrococcus furiosus
J. Agric. Food Chem.
61
11532-11540
2013
-
1
1
-
-
-
8
4
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2
-
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1
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-
8
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8
1
1
1
5
4
1
1
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1
1
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8
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4
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2
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8
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8
1
1
1
5
4
1
1
-
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-
-
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-
4
4
721389
Böhmer
Recombinant production of hype ...
Pyrococcus furiosus
Appl. Microbiol. Biotechnol.
96
903-912
2012
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1
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1
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721908
Nam
Quercetin production from ruti ...
Pyrococcus furiosus
Biotechnol. Lett.
34
483-489
2012
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2
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2
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2
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2
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3
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1
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5
2
1
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722435
Yeom
Hydrolysis of isoflavone glyco ...
Pyrococcus furiosus
J. Agric. Food Chem.
60
1535-1541
2012
1
-
1
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1
3
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1
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1
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1
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4
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4
1
1
1
6
3
1
1
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1
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1
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1
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3
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1
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1
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4
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4
1
1
1
6
3
1
1
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723242
Park
Expression and characterizatio ...
Pyrococcus furiosus
N. Biotechnol.
28
639-648
2011
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2
1
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5
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3
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1
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1
1
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5
5
1
1
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2
1
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5
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3
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1
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9
1
1
1
5
5
1
1
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5
5
735377
Kado
Structure of hyperthermophilic ...
Pyrococcus furiosus
Acta Crystallogr. Sect. F
67
1473-1479
2011
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1
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1
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735531
Yoo
Production of aglycon protopan ...
Pyrococcus furiosus
Appl. Microbiol. Biotechnol.
89
1019-1028
2011
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9
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11
1
1
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1
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1
1
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1
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9
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11
1
1
-
1
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1
1
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736532
Mayer
Continuous production of lactu ...
Pyrococcus furiosus
J. Biotechnol.
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387-393
2010
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735803
Schwarz
Enzymatic synthesis of beta-gl ...
Pyrococcus furiosus
Biotechnol. Bioeng.
103
865-872
2009
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1
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1
-
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-
-
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-
736073
Powers
Denaturation of an extremely s ...
Pyrococcus furiosus
Extremophiles
11
179-189
2007
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-
-
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1
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1
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736531
Kamrat
Entrapment in E. coli improves ...
Pyrococcus furiosus
J. Biotechnol.
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69-76
2007
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1
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1
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1
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721889
Lang
Influence of ionic liquid coso ...
Pyrococcus furiosus
Biotechnol. Bioeng.
95
1093-1100
2006
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-
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1
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1
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1
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1
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1
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-
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-
-
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736650
Bruins
-
Equilibrium shifts in enzyme r ...
Pyrococcus furiosus
J. Mol. Catal. B
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124-127
2006
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-
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1
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-
-
1
-
-
-
-
-
-
-
-
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737309
Lin
Overexpression of celB gene co ...
Pyrococcus furiosus
Z. Naturforsch. C
61
595-600
2006
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1
1
-
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-
-
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1
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1
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1
-
1
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1
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1
1
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-
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-
1
-
1
-
1
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1
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-
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-
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-
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735665
Perugino
Activity of hyperthermophilic ...
Pyrococcus furiosus
Biochemistry
42
8484-8493
2003
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-
-
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1
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6
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1
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10
-
6
-
-
-
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6
-
-
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-
-
-
1
-
-
-
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6
-
-
-
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-
-
-
-
10
-
6
-
-
-
-
6
-
-
-
-
-
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-
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6
6
735813
Bruins
Maillard reactions and increas ...
Pyrococcus furiosus
Biotechnol. Bioeng.
81
546-552
2003
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1
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2
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1
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1
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1
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1
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1
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654641
Kaper
Substrate specificity engineer ...
Pyrococcus furiosus
Biochemistry
41
4147-4155
2002
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1
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3
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1
15
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1
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1
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3
-
-
-
2
15
-
-
-
-
4
-
-
-
-
1
-
-
3
-
-
1
4
15
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
2
15
-
-
-
-
-
-
-
-
15
15
724155
Hansson
-
Enzymatic synthesis of hexyl g ...
Pyrococcus furiosus
Biocatal. Biotransform.
20
167-178
2002
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1
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-
-
-
-
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1
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5
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3
-
1
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-
1
-
-
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-
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-
1
-
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-
-
-
-
-
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-
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-
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-
-
-
5
-
3
-
1
-
-
-
1
-
-
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724212
Kaper
DNA family shuffling of hypert ...
Pyrococcus furiosus
Biochem. J.
368
461-470
2002
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1
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1
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2
4
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1
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4
-
2
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2
4
1
-
-
-
2
-
-
-
-
1
-
-
1
-
-
2
2
4
-
-
-
-
-
-
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1
-
-
-
-
4
-
2
-
2
4
1
-
-
-
-
-
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-
4
4
724625
Petzelbauer
Development of an ultrahigh-te ...
Pyrococcus furiosus
Biotechnol. Bioeng.
77
394-404
2002
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1
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1
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1
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1
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1
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-
-
1
-
-
-
-
-
-
-
-
-
-
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724626
Petzelbauer
Development of an ultrahigh-te ...
Pyrococcus furiosus
Biotechnol. Bioeng.
77
619-631
2002
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2
-
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1
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4
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1
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-
1
-
-
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1
-
1
-
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-
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-
-
2
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-
-
1
-
-
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4
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
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-
-
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724633
Hansson
-
The temperature influences the ...
Pyrococcus furiosus
Biotechnol. Lett.
24
1465-1471
2002
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-
-
-
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6
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2
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1
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-
-
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-
-
-
-
-
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6
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-
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2
-
1
-
-
-
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-
-
-
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735469
Splechtna
Hydrolysis of lactose by beta- ...
Pyrococcus furiosus
Appl. Biochem. Biotechnol.
98-100
473-88
2002
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1
1
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1
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1
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1
1
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-
-
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-
-
-
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-
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-
1
-
-
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-
-
-
-
-
-
-
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721888
Hansson
Enhanced transglucosylation/hy ...
Pyrococcus furiosus
Biotechnol. Bioeng.
75
656-665
2001
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1
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3
-
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4
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1
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1
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1
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2
1
1
-
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4
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
4
-
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1
-
-
-
-
1
-
-
-
-
2
1
1
-
-
4
-
-
-
-
-
-
-
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-
724870
Hansson
-
Influence of water activity on ...
Pyrococcus furiosus
Enzyme Microb. Technol.
29
527-534
2001
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-
-
-
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1
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1
1
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-
-
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4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
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-
1
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-
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4
-
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-
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-
-
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326270
Kaper
Comparative structural analysi ...
Pyrococcus furiosus
Biochemistry
39
4963-4970
2000
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1
4
-
-
14
-
-
-
-
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1
-
-
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-
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-
-
-
3
-
-
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-
14
3
-
-
-
-
-
-
-
-
-
-
1
4
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
14
3
-
-
-
-
-
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14
14
722169
Petzelbauer
Transgalactosylation by thermo ...
Pyrococcus furiosus
Eur. J. Biochem.
267
5055-5066
2000
-
-
-
-
-
-
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3
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1
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-
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-
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-
-
4
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
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4
-
-
-
-
3
-
-
-
-
-
-
-
-
3
3
722214
Pouwels
Activity and stability of hype ...
Pyrococcus furiosus
Extremophiles
4
157-164
2000
-
-
1
-
-
2
1
10
-
-
-
-
-
1
-
-
1
-
-
-
-
-
4
-
-
-
1
10
1
-
1
-
-
-
-
-
-
1
-
-
-
2
-
1
-
10
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
1
10
1
-
1
-
-
-
-
-
-
-
735663
Lebbink
Improving low-temperature cata ...
Pyrococcus furiosus
Biochemistry
39
3656-3665
2000
-
-
-
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9
-
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27
-
-
-
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-
1
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-
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-
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-
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4
-
1
-
1
26
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
27
-
-
-
-
-
-
-
-
-
-
-
-
4
-
1
-
1
26
-
-
-
-
-
-
-
-
26
26
724623
Petzelbauer
Development of an ultra-high-t ...
Pyrococcus furiosus
Biotechnol. Bioeng.
64
322-332
1999
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1
1
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-
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2
2
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1
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-
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-
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3
-
1
-
4
-
1
-
-
-
2
-
-
-
1
1
-
-
-
-
-
2
2
2
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
4
-
1
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-
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736342
Voorhorst
Transcriptional regulation in ...
Pyrococcus furiosus
J. Bacteriol.
181
3777-3783
1999
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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2
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2
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722489
Voorhorst
Characterization of the celB g ...
Pyrococcus furiosus
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1995
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2
1
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1
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1
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-
2
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-
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2
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1
-
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1
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-
-
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2
1
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-
1
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722161
Kengen
Purification and characterizat ...
Pyrococcus furiosus
Eur. J. Biochem.
213
305-312
1993
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2
2
1
2
2
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1
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1
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-
-
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4
1
1
1
2
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
2
1
2
1
2
2
-
-
-
-
1
-
-
-
-
4
1
1
1
2
1
1
-
-
1
-
-
-
-
-
-