Literature summary for 3.2.1.B28 extracted from

Petzelbauer, I.; Splechtna, B.; Nidetzky, B.
Development of an ultrahigh-temperature process for the enzymatic hydrolysis of lactose. III. Utilization of two thermostable beta-glycosidases in a continuous ultrafiltration membrane reactor and galacto-oligosaccharide formation under steady-state conditions (2002), Biotechnol. Bioeng., 77, 394-404.

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Application

Application	Comment	Organism
synthesis	a continuous stirred-tank reactor charged with the enzyme and operated at steady-state conditions could be a useful reaction system for the production of galacto-oligosaccharides in which composition is narrower and more easily programmable, in terms of the individual components contained, as compared to the batchwise reaction	Pyrococcus furiosus

General Stability

General Stability	Organism
half-life times of the enzyme in hydrolysis of lactose is carried out at 70°C in a continuous stirred-tank reactor coupled to a 10000 Da cross-flow ultrafiltration module (to recycle the enzyme) is approximately 5 to 7 days	Pyrococcus furiosus

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus furiosus	Q51723	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
lactose + H2O	-	Pyrococcus furiosus	D-glucose + D-galactose	-	?

Synonyms

Synonyms	Comment	Organism
CelB	-	Pyrococcus furiosus

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Release 2023.1 (January 2023)