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Literature summary for 3.2.1.B28 extracted from
- Development of an ultra-high-temperature process for the enzymatic hydrolysis of lactose. I. The properties of two thermostable beta-glycosidases (1999), Biotechnol. Bioeng., 64, 322-332.
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | the enzyme suitable for hydrolysis of lactose at temperatures at 70-80°C | Pyrococcus furiosus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 | Pyrococcus furiosus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-galactose | measured with lactose as the substrate | Pyrococcus furiosus |  |
| D-glucose | measured with 2-nitrophenyl beta-D-galactoside as the substrate | Pyrococcus furiosus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.3 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 5.5, 80°C | Pyrococcus furiosus | |
| 107 | - |
lactose | pH 5.5, 80°C | Pyrococcus furiosus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | Q51723 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Pyrococcus furiosus | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
| lactose + H2O | - |
Pyrococcus furiosus | D-glucose + D-galactose | - |
? | |
| additional information | the transfer of the galactosyl group from lactose to acceptors such as lactose or D-glucose rather than water is significant and depends on the initial lactose concentration as well as the time-dependent substrate/product ratio during batchwise lactose conversion | Pyrococcus furiosus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CelB | - |
Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
assay at | Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermostability is not affected by the presence of Mg2+ | Pyrococcus furiosus |
| 70 | - |
t1/2: 60 d | Pyrococcus furiosus |
| 80 | - |
t1/2: about 22 d | Pyrococcus furiosus |
| 90 | - |
t1/2: 3.2 d | Pyrococcus furiosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
assay at | Pyrococcus furiosus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 76 | - |
D-glucose | pH 5.5, 80°C | Pyrococcus furiosus | |
| 270 | - |
D-galactose | pH 5.5, 80°C | Pyrococcus furiosus | |
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Release 2023.1 (January 2023)
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