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Literature summary for 3.2.1.B28 extracted from

  • Kaper, T.; Brouns, S.J.; Geerling, A.C.; De Vos, W.M.; Van der Oost, J.
    DNA family shuffling of hyperthermostable beta-glycosidases (2002), Biochem. J., 368, 461-470.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.B28

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus

Protein Variants

Protein Variants Comment Organism
additional information generation of functional glycoside hydrolase hybrids by shuffling of the genes coding for Pyrococcus furiosus CelB and Sulfolobus solfataricus LacS. Generating of thermostable hybrid beta-glycosidases and isolating high-performance variants Pyrococcus furiosus

Inhibitors

Inhibitors Comment Organism Structure
(5R,6R,7S,8S)-5-(hydroxymethyl)-2-phenyl-5,6,7,8-tetrahydroimidazol[1,2-a]pyridine-6,7,8-triol inhibits activity with 4-nitrophenyl beta-D-galactopyranoside Pyrococcus furiosus
D-glucose inhibits activity with 4-nitrophenyl beta-D-galactopyranoside Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 70°C Pyrococcus furiosus
2.6
-
 4-nitrophenyl beta-D-galactopyranoside pH 5.0, 70°C Pyrococcus furiosus
3.2
-
 cellobiose pH 5.0, 70°C Pyrococcus furiosus
59.8
-
 lactose pH 5.0, 70°C Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q51723
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-galactopyranoside + H2O CelB is a little more active on the galactosides lactose and 4-nitrophenyl beta-D-galactopyranoside than on 4-nitrophenyl beta-D-glucopyranoside, whereas the efficiency of cellobiose hydrolysis is relatively low Pyrococcus furiosus 4-nitrophenol + D-galactose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O CelB is a little more active on the galactosides lactose and 4-nitrophenyl beta-D-galactopyranoside than on 4-nitrophenyl beta-D-glucopyranoside, whereas the efficiency of cellobiose hydrolysis is relatively low Pyrococcus furiosus 4-nitrophenol + D-glucopyranose
-
 ?
cellobiose + H2O CelB is a little more active on the galactosides lactose and 4-nitrophenyl beta-D-galactopyranoside than on 4-nitrophenyl beta-D-glucopyranoside, whereas the efficiency of cellobiose hydrolysis is relatively low Pyrococcus furiosus D-glucose + D-glucose
-
 ?
lactose + H2O CelB is a little more active on the galactosides lactose and 4-nitrophenyl beta-D-galactopyranoside than on 4-nitrophenyl beta-D-glucopyranoside, whereas the efficiency of cellobiose hydrolysis is relatively low Pyrococcus furiosus D-glucose + D-galactose
-
 ?

Synonyms

Synonyms Comment Organism
CelB
-
 Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Pyrococcus furiosus
104
-
-
 Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
92
-
 half-life: above 100 min Pyrococcus furiosus
104
-
 Tm-value Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
37.6
-
 cellobiose pH 5.0, 70°C Pyrococcus furiosus
206
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 70°C Pyrococcus furiosus
286
-
 lactose pH 5.0, 70°C Pyrococcus furiosus
298
-
 4-nitrophenyl beta-D-galactopyranoside pH 5.0, 70°C Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 assay at Pyrococcus furiosus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000093
-
 (5R,6R,7S,8S)-5-(hydroxymethyl)-2-phenyl-5,6,7,8-tetrahydroimidazol[1,2-a]pyridine-6,7,8-triol pH 5.0, 70°C, substrate: 4-nitrophenyl beta-D-galactopyranoside Pyrococcus furiosus
43
-
 D-glucose pH 5.0, 70°C, substrate: 4-nitrophenyl beta-D-galactopyranoside Pyrococcus furiosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.78
-
 lactose pH 5.0, 70°C Pyrococcus furiosus
11.8
-
 cellobiose pH 5.0, 70°C Pyrococcus furiosus
115
-
 4-nitrophenyl beta-D-galactopyranoside pH 5.0, 70°C Pyrococcus furiosus
258
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 70°C Pyrococcus furiosus