Application | Comment | Organism |
---|---|---|
food industry | the hyperthermostable beta-glycosidase may be useful for food and pharmaceutical applications | Pyrococcus furiosus |
pharmacology | the hyperthermostable beta-glycosidase may be useful for food and pharmaceutical applications | Pyrococcus furiosus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
lactose | pH 5.0, 100°C | Pyrococcus furiosus | |
0.07 | - |
cellobiose | pH 5.0, 100°C | Pyrococcus furiosus | |
0.09 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 100°C | Pyrococcus furiosus | |
0.09 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 100°C | Pyrococcus furiosus | |
0.12 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 5.0, 100°C | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56326 | - |
4 * 56326, calculated from sequence | Pyrococcus furiosus |
57000 | - |
4 * 57000, SDS-PAGE | Pyrococcus furiosus |
230000 | - |
non-denaturing PAGE | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8U3U9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Pyrococcus furiosus | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | 37% of the activity compared to 4-nitrophenyl-beta-D-mannopyranoside | Pyrococcus furiosus | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | 114% of the activity compared to 4-nitrophenyl-beta-D-mannopyranoside | Pyrococcus furiosus | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-mannopyranoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + beta-D-mannopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | 37% of the activity compared to 4-nitrophenyl-beta-D-mannopyranoside | Pyrococcus furiosus | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
cellobiose + H2O | - |
Pyrococcus furiosus | 2 beta-D-glucose | - |
? | |
lactose + H2O | - |
Pyrococcus furiosus | D-glucose + D-galactose | - |
? | |
mannooligosaccharide + H2O | hydrolysis of mannose from the reducing end | Pyrococcus furiosus | ? | - |
? | |
additional information | the enzyme shows transglycosylation and transgalactosylation activities toward cellobiose, lactose and mannooligosaccharides that could produce galactooligosaccharides and maltooligosaccharides. It can not hydrolyze alpha-form substrates and bulk polymers like mannan and starch | Pyrococcus furiosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 57000, SDS-PAGE | Pyrococcus furiosus |
tetramer | 4 * 56326, calculated from sequence | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
PFTG | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
- |
Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 110 | 70°C: about 50% of maximal activity, 110°C: about 45% of maximal activity | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
2 h, stable | Pyrococcus furiosus |
90 | - |
half-life: 398 min | Pyrococcus furiosus |
95 | - |
half-life: 114 min | Pyrococcus furiosus |
100 | - |
1 h, 73% loss of activity | Pyrococcus furiosus |
110 | - |
thermal transition midpoint | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
lactose | pH 5.0, 100°C | Pyrococcus furiosus | |
0.16 | - |
cellobiose | pH 5.0, 100°C | Pyrococcus furiosus | |
0.23 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 5.0, 100°C | Pyrococcus furiosus | |
0.26 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 100°C | Pyrococcus furiosus | |
0.3 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 100°C | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
sodium citrate buffer | Pyrococcus furiosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 6 | pH 4.5: about 85% of maximal activity, pH 6.0: about 60% of maximal activity | Pyrococcus furiosus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.82 | - |
lactose | pH 5.0, 100°C | Pyrococcus furiosus | |
1.89 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 5.0, 100°C | Pyrococcus furiosus | |
2.28 | - |
cellobiose | pH 5.0, 100°C | Pyrococcus furiosus | |
2.9 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 100°C | Pyrococcus furiosus | |
3.02 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 5.0, 100°C | Pyrococcus furiosus |