Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose | weak | Pyrococcus furiosus | |
SDS | 0.1%, activity is completely and irreversibly blocked | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5, 80°C | Pyrococcus furiosus | |
20 | - |
cellobiose | pH 5, 80°C | Pyrococcus furiosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Pyrococcus furiosus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | divalent metal ions not required | Pyrococcus furiosus | |
NaCl | optimal concentration: 200 mM | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
4 * 58000, SDD-PAGE | Pyrococcus furiosus |
230000 | - |
non-denaturing PAGE | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-galactopyranoside + H2O | hydrolysed at 34% compared to activity with 4-nitrophenyl-beta-D-glucopyranoside | Pyrococcus furiosus | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | hydrolysed at 9.2% compared to activity with 4-nitrophenyl-beta-D-glucopyranoside | Pyrococcus furiosus | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
cellobiose + H2O | as active as 4-nitrophenyl-beta-D-glucopyranoside | Pyrococcus furiosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 58000, SDD-PAGE | Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
102 | 105 | - |
Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 110 | 80°C: about 45% of maximal activity, 110°C: about 65% of maximal activity | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
half-life: 85 h | Pyrococcus furiosus |
110 | - |
half-life: 13 h | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
454 | - |
cellobiose | pH 5, 80°C | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Pyrococcus furiosus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
300 | - |
D-glucose | pH 5, 80°C | Pyrococcus furiosus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pyrococcus furiosus | isoelectric focusing | - |
4.4 |