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Literature summary for 3.2.1.B1 extracted from
- Secretory expression and enzymatic characterization of recombinant Agarivorans albus beta-agarase in Escherichia coli (2017), Prep. Biochem. Biotechnol., 47, 1037-1042 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21-Star(DE3) cells | Agarivorans albus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | strong inhibition | Agarivorans albus |  |
| Cu2+ | strong inhibition | Agarivorans albus | |
| Mn2+ | strong inhibition | Agarivorans albus | |
| additional information | not influenced by EDTA, 2-mercaptoethanol, dithiothreitol, and urea | Agarivorans albus | |
| Zn2+ | strong inhibition | Agarivorans albus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
agarose | at pH 7.0 and 25°C | Agarivorans albus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Agarivorans albus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | neither significant activation nor inhibition in the presence of Na+, K+, Ca2+, Ba2+, and Mg2+ | Agarivorans albus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agarose + H2O | Agarivorans albus | 100% activity | neoagarohexaose + neoagarotetraose + D-galactose | - |
? | |
| carboxymethyl cellulose + H2O | Agarivorans albus | 1.4% activity compared to agarose | ? | - |
? | |
| starch + H2O | Agarivorans albus | 1.8% activity compared to agarose | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agarivorans albus | A8W969 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation and DEAE Sepharose column chromatography | Agarivorans albus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 29.48 | - |
crude extract, at pH 7.0 and 25°C | Agarivorans albus |
| 265.32 | - |
after 9fold purification, at pH 7.0 and 25°C | Agarivorans albus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agarose + H2O | 100% activity | Agarivorans albus | neoagarohexaose + neoagarotetraose + D-galactose | - |
? | |
| carboxymethyl cellulose + H2O | 1.4% activity compared to agarose | Agarivorans albus | ? | - |
? | |
| starch + H2O | 1.8% activity compared to agarose | Agarivorans albus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Agarivorans albus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-agarase | - |
Agarivorans albus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Agarivorans albus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | the enzyme retains more than 80% of its maximal activity within a temperature range of 30°C to 50°C | Agarivorans albus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 50 | the enzyme is thermostable at temperatures from 35-50°C with more than 80% activity remaining after 30 min incubation | Agarivorans albus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 45 | - |
agarose | at pH 7.0 and 25°C | Agarivorans albus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Agarivorans albus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | the enzyme retains more than 70% of its maximal activity within a pH range of 5.0-9.0 | Agarivorans albus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | the enzyme exhibits more than 80% of its maximal activity over a pH range of 5.0 to 9.0 after 1 h incubation and shows more than 40% of maximal activity at pH values below 4.0 and above 11.0 | Agarivorans albus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2250 | - |
agarose | at pH 7.0 and 25°C | Agarivorans albus | |
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