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Literature summary for 3.2.1.B1 extracted from

  • Li, L.; Qu, W.; Jin, M.; Di, W.; Zeng, R.
    Extracellular expression of agarase rAgaM1 in Bacillus subtilis and its ability for neoagaro-oligosaccharide production (2019), J. Basic Microbiol., 59, 359-367 .
    View publication on PubMed
Search for more literature for 3.2.1.B1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bacillus subtilis
-
-

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis WB800N
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarose + H2O
-
 Bacillus subtilis neoagarohexaose + neoagarotetraose
-
 ?
agarose + H2O
-
 Bacillus subtilis WB800N neoagarohexaose + neoagarotetraose
-
 ?
Gracilaria lemaneiformis + H2O
-
 Bacillus subtilis neoagarohexaose + neoagarotetraose
-
 ?
Gracilaria lemaneiformis + H2O
-
 Bacillus subtilis WB800N neoagarohexaose + neoagarotetraose
-
 ?

Subunits

Subunits Comment Organism
? x * 85000, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
agaM1
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 80 91.9% and 84.5% of activity are retained after pre-incubation at 40°C and 50°C for 2 h, respectively. 62.5% of the enzyme activity is lost after pre-incubation at 60°C for 2 h, and the enzyme activity is completely lost after pre-incubation at 70°C and 80°C Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Bacillus subtilis