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Literature summary for 3.2.1.B1 extracted from
- Purification and characterization of thermostable agarase from Bacillus sp. BI-3, a thermophilic bacterium isolated from hot spring (2014), J. Microbiol. Biotechnol., 24, 19-25.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 50% residual activity at 2 mM | Bacillus sp. (in: Bacteria) |  |
| Co2+ | complete inhibition at 2mM | Bacillus sp. (in: Bacteria) | |
| Cu2+ | complete inhibition at 2mM | Bacillus sp. (in: Bacteria) | |
| EDTA | complete inhibition at 2mM | Bacillus sp. (in: Bacteria) | |
| Fe2+ | complete inhibition at 2mM | Bacillus sp. (in: Bacteria) | |
| Mn2+ | complete inhibition at 2mM | Bacillus sp. (in: Bacteria) | |
| Zn2+ | complete inhibition at 2mM | Bacillus sp. (in: Bacteria) | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 150% activity at 2 mM | Bacillus sp. (in: Bacteria) | |
| K+ | 250% activity at 2 mM | Bacillus sp. (in: Bacteria) | |
| Mg2+ | 125% activity at 2 mM | Bacillus sp. (in: Bacteria) | |
| Na+ | 375% activity at 2 mM | Bacillus sp. (in: Bacteria) | |
| Sr2+ | 400% activity at 2 mM | Bacillus sp. (in: Bacteria) | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
x * 58000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agarose + H2O | Bacillus sp. (in: Bacteria) | - |
neoagarobiose + neoagarohexaose + neoagarotetraose | neoagarobiose is the main product | ? | |
| agarose + H2O | Bacillus sp. (in: Bacteria) BI-3 | - |
neoagarobiose + neoagarohexaose + neoagarotetraose | neoagarobiose is the main product | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | - |
- |
- |
| Bacillus sp. (in: Bacteria) BI-3 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, Q-Sepharose column chromatography and Sephacryl S-200 gel filtration | Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agarose + H2O | - |
Bacillus sp. (in: Bacteria) | neoagarobiose + neoagarohexaose + neoagarotetraose | neoagarobiose is the main product | ? | |
| agarose + H2O | - |
Bacillus sp. (in: Bacteria) BI-3 | neoagarobiose + neoagarohexaose + neoagarotetraose | neoagarobiose is the main product | ? | |
| neoagarohexaitol + H2O | the enzyme the cleaves the beta-1,4 linkage near a sugar alcohol in neoagarohexaitol | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| neoagarohexaitol + H2O | the enzyme the cleaves the beta-1,4 linkage near a sugar alcohol in neoagarohexaitol | Bacillus sp. (in: Bacteria) BI-3 | ? | - |
? | |
| neoagarohexaose + H2O | - |
Bacillus sp. (in: Bacteria) | neoagarotetraose + neoagarobiose | - |
? | |
| neoagarohexaose + H2O | - |
Bacillus sp. (in: Bacteria) BI-3 | neoagarotetraose + neoagarobiose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 58000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 80 | the enzyme activity consistently increases from 55°C to 70°C with optimum activity at 70°C, and an obvious decrease is observed when the agarase is incubated at temperatures above 80°C | Bacillus sp. (in: Bacteria) |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
more than 50% activity is retained at 80°C for 15 min | Bacillus sp. (in: Bacteria) |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | more than 60% of the residual activity is retained in the pH range of 5.8-8.0 | Bacillus sp. (in: Bacteria) |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 2 | - |
at pH 6.5 and 70°C | Bacillus sp. (in: Bacteria) | Ba2+ | |
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