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Literature summary for 3.2.1.99 extracted from

  • Machado, C.B.; Citadini, A.P.; Goldbeck, R.; de Lima, E.A.; Figueiredo, F.L.; da Silva, T.M.; Hoffmam, Z.B.; de Sousa, A.S.; Squina, F.M.; de Lourdes Teixeira de Moraes Polizeli, M.; Ruller, R.; Ward, R.J.
    Increased biomass saccharification by supplementation of a commercial enzyme cocktail with endo-arabinanase from Bacillus licheniformis (2015), Biotechnol. Lett., 37, 1455-1462 .
    View publication on PubMed
Search for more literature for 3.2.1.99

Application

Application Comment Organism
additional information the hydrolysis of pretreated sugar cane biomass increases by 15% when a commercial enzyme cocktail is supplemented with immobilized ABNase. Pectin hydrolysis by recombinant ABNase plays a role in the effective application of enzymatic cocktails for biomass saccharification Bacillus licheniformis

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bacillus licheniformis

General Stability

General Stability Organism
the enzyme stability increases 128fold when immobilized with glyoxyl agarose Bacillus licheniformis

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 5 mM, complete inhibition Bacillus licheniformis
EDTA 10 mM, 58% loss of activity Bacillus licheniformis
EGTA 10 mM, 30% loss of activity Bacillus licheniformis
Fe3+ 5 mM, complete inhibition Bacillus licheniformis
Zn2+ 5 mM, complete inhibition Bacillus licheniformis

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 5 mM, 1.1fold activation Bacillus licheniformis
KCl 5 mM, 1.1fold activation Bacillus licheniformis
LiCl 5 mM, 1.2fold activation Bacillus licheniformis
MgCl2 5 mM, 1.3fold activation Bacillus licheniformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
-
 Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus licheniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
branched arabinan + H2O branched arabinan from sugar beet. Hydrolyzed at 12% compared to debranched arabinan Bacillus licheniformis ?
-
 ?
debranched arabinan + H2O
-
 Bacillus licheniformis ?
-
 ?
linear arabinan + H2O hydrolyzed at 62% compared to debranched arabinan Bacillus licheniformis ?
-
 ?
additional information no activity with: laminarin, lichenan, galactomannan, beta-glucan, wheat and rye arabinoxylan, xyloglucan, carboxymethyl cellulose, pectin from fruits, larch arabinogalactan, galactan (potato), xylan from beechwood, and 1,4-beta-mannan Bacillus licheniformis ?
-
 ?

Synonyms

Synonyms Comment Organism
ABNase
-
 Bacillus licheniformis
endo-arabinanase
-
 Bacillus licheniformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40
-
 Bacillus licheniformis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 45 30°C: about 75% of maximal activity, 45°C: about 70% of maximal activity Bacillus licheniformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 50% loss of activity after 30 min incubation at 50°C. After 60 min the activity is about 20% of the initial value Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6.5
-
 Bacillus licheniformis

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5 pH 5.0: about 65% of maximal activity, pH 8.5: about 40% of maximal activity Bacillus licheniformis