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Literature summary for 3.2.1.99 extracted from
- Structures of endo-1,5-alpha-L-arabinanase mutants from Bacillus thermodenitrificans TS-3 in complex with arabino-oligosaccharides (2018), Acta Crystallogr. Sect. F, 74, 774-780 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21(DE3) cells as fusion proteins with a His-tag at the C-terminus | Geobacillus thermodenitrificans |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting-drop vapor-diffusion method at 20°C. Crystal structures of inactive mutant enzymes D27A and D147N, are determined in complex with arabino-oligosaccharides | Geobacillus thermodenitrificans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D147N | inactive mutant enzyme | Geobacillus thermodenitrificans |
| D27A | inactive mutant enzyme | Geobacillus thermodenitrificans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus thermodenitrificans | Q93HT9 | - |
- |
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Release 2023.1 (January 2023)
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