Literature summary for 3.2.1.99 extracted from

Squina, F.; Prade, R.; Wang, H.; Murakami, M.
Expression, purification, crystallization and preliminary crystallographic analysis of an endo-1,5-alpha-L-arabinanase from hyperthermophilic Thermotoga petrophila (2009), Acta Crystallogr. Sect. F, 65, 902-905 .

Application

Application	Comment	Organism
biofuel production	conversion of lignocellulosic biomass to biofuels. Endo-1,5-alpha-L-arabinanase hydrolyzes alpha-1,5-arabinofuranosidic bonds in hemicelluloses such as arabinoxylan and arabinan as well as in other arabinose-containing polysaccharides	Thermotoga petrophila

Cloned(Commentary)

Cloned (Comment)	Organism
overexpressed in Escherichia coli Top10	Thermotoga petrophila

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystallization is performed by the sitting-drop vapour-diffusion method. Single crystals are obtained from a solution containing 0.1 M MES buffer pH 6.5, 0.8 M ammonium sulfate, 0.1 M EDTA, 0.1 M L-proline and 5% (v/v) dioxane. X-ray diffraction data are collected to a resolution of 2.86 A using synchrotron radiation. The diffraction pattern is indexed in the tetragonal space group P422, with unit-cell parameters a = b = 83.71, c = 408.25 A	Thermotoga petrophila

Crystallization (Comment)

Organism

crystallization is performed by the sitting-drop vapour-diffusion method. Single crystals are obtained from a solution containing 0.1 M MES buffer pH 6.5, 0.8 M ammonium sulfate, 0.1 M EDTA, 0.1 M L-proline and 5% (v/v) dioxane. X-ray diffraction data are collected to a resolution of 2.86 A using synchrotron radiation. The diffraction pattern is indexed in the tetragonal space group P422, with unit-cell parameters a = b = 83.71, c = 408.25 A

Thermotoga petrophila

Organism

Organism	UniProt	Comment	Textmining
Thermotoga petrophila	A5IKD4	-	-
Thermotoga petrophila DSM 13995	A5IKD4	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermotoga petrophila

Synonyms

Synonyms	Comment	Organism
Tpet_0637	-	Thermotoga petrophila

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Release 2023.1 (January 2023)