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Literature summary for 3.2.1.99 extracted from
- Purification and characterization of an endo-D-arabinase produced by Cellulomonas (2012), Protein J., 31, 51-58.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0556 | - |
arabinogalactan | at pH 8.0 and 40°C | Cellulomonas sp. |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, SDS-PAGE | Cellulomonas sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arabinogalactan + H2O | Cellulomonas sp. | - |
hexa-alpha-(1->5)-arabinofuranoside + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cellulomonas sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE Sepharose column chromatography, Sephacryl S-300 gel filtration, and Blue Sepharose affinity chromatography | Cellulomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arabinogalactan + H2O | - |
Cellulomonas sp. | hexa-alpha-(1->5)-arabinofuranoside + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Cellulomonas sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CEDAase | - |
Cellulomonas sp. |
| endo-D-arabinase | - |
Cellulomonas sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Cellulomonas sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
no obvious enzymatic activity decline is observed for enzyme incubated at 40°C for 120 min. The enzyme retains the most of its initial enzyme activity at 50°C for a 120 min incubation. However, the activity decreases rapidly following its incubation at 60°C | Cellulomonas sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Cellulomonas sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | the enzyme remains stable at pH 6.0-9.0 after 24 h incubation | Cellulomonas sp. |
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