Literature summary for 3.2.1.99 extracted from

McVey, C.E.; Ferreira, M.J.; Correia, B.; Lahiri, S.; de Sanctis, D.; Carrondo, M.A.; Lindley, P.F.; de Sa Nogueira, I.; Soares, C.M.; Bento, I.
The importance of the Abn2 calcium cluster in the endo-1,5-arabinanase activity from Bacillus subtilis (2014), J. Biol. Inorg. Chem., 19, 505-513.

Search for more literature for 3.2.1.99

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) pLysS cells	Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment)	Organism
vapor diffusion method, using 10 mM NiCl2, 100 mM tris(hydroxymethyl)aminomethane-HCl pH 8.5, and 20% PEG 2000 monomethyl ether	Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
H318Q	inactive, contains Ni2+ instead of Ca2+	Bacillus subtilis

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	-	Bacillus subtilis
EGTA	-	Bacillus subtilis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	the enzyme contains Ca2+ which also stimulates activity	Bacillus subtilis
Mn2+	stimulates activity	Bacillus subtilis
Ni2+	stimulates activity	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	P42293	-	-
Bacillus subtilis 168	P42293	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1,5-alpha-L-arabinan + H2O	-	Bacillus subtilis	oligo-alpha-(1->5)-L-arabinoside + L-arabinose	-	?
1,5-alpha-L-arabinan + H2O	-	Bacillus subtilis 168	oligo-alpha-(1->5)-L-arabinoside + L-arabinose	-	?
sugar beet arabinan + H2O	-	Bacillus subtilis	oligo-alpha-(1->5)-L-arabinoside + L-arabinose	-	?
sugar beet arabinan + H2O	-	Bacillus subtilis 168	oligo-alpha-(1->5)-L-arabinoside + L-arabinose	-	?

Synonyms

Synonyms	Comment	Organism
Abn2	-	Bacillus subtilis
endo-1,5-arabinase	-	Bacillus subtilis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)