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Literature summary for 3.2.1.99 extracted from

  • Huy, N.D.; Thiyagarajan, S.; Choi, Y.E.; Kim, D.H.; Park, S.M.
    Cloning and characterization of a thermostable endo-arabinanase from Phanerochaete chrysosporium and its synergistic action with endo-xylanase (2013), Bioprocess Biosyst. Eng., 36, 677-685.
    View publication on PubMed
Search for more literature for 3.2.1.99

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain GS115 Phanerodontia chrysosporium

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ about 40% residual activity at 5 mM Phanerodontia chrysosporium
Co2+ about 70% residual activity at 5 mM Phanerodontia chrysosporium
Cu2+ about 10% residual activity at 5 mM Phanerodontia chrysosporium
Fe2+ about 60% residual activity at 5 mM Phanerodontia chrysosporium
Fe3+ about 55% residual activity at 5 mM Phanerodontia chrysosporium
Mg2+ about 80% residual activity at 5 mM Phanerodontia chrysosporium
Ni2+ about 70% residual activity at 5 mM Phanerodontia chrysosporium
Zn2+ about 40% residual activity at 5 mM Phanerodontia chrysosporium

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not influenced by K+ and Mn2+ Phanerodontia chrysosporium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 x * 33000, SDS-PAGE Phanerodontia chrysosporium
33900
-
 x * 33900, calculated from amino acid sequence Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium I6XPK9
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ His-tag column chromatography Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
debranched arabinan + H2O highest activity Phanerodontia chrysosporium oligo-alpha-(1->5)-L-arabinoside
-
 ?
additional information does not hydrolyze birchwood xylan and 4-nitrophenyl-alpha-L-arabinofuranoside Phanerodontia chrysosporium ?
-
 ?
sugar beet arabinan + H2O
-
 Phanerodontia chrysosporium ?
-
 ?
wheat arabinoxylan + H2O lowest activity Phanerodontia chrysosporium ?
-
 ?

Subunits

Subunits Comment Organism
? x * 33000, SDS-PAGE Phanerodontia chrysosporium
? x * 33900, calculated from amino acid sequence Phanerodontia chrysosporium

Synonyms

Synonyms Comment Organism
ARA
-
 Phanerodontia chrysosporium
endo-arabinanase
-
 Phanerodontia chrysosporium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Phanerodontia chrysosporium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80 the enzyme shows less than 40% activity at 30°C, less than 50% activity at 40°C, and more than 80% activity at 50°V. Enzyme activity decreases rapidly when the reaction temperature is above 60°C, and the enzyme loses most of ist activity at 80°C Phanerodontia chrysosporium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Phanerodontia chrysosporium

pH Range

pH Minimum pH Maximum Comment Organism
3 8 the enzyme shows 30%, 55%, 100%, 70%, 15% and less than 5% activity at pH 3.0, 4.0, 5.0, 6.0, 7.0, and 8.0, respectively Phanerodontia chrysosporium