Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) pLysS cells | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 65% (w/v) 2-methyl-2,4-pentadiol and 100 mM Tris, pH 8.5 | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
D171A | the mutant of isoform Arb43B displays no measurable activity | Bacillus subtilis |
D38A | the mutant of isoform Arb43B displays no measurable activity | Bacillus subtilis |
E224A | the mutant of isoform Arb43B displays no measurable activity | Bacillus subtilis |
H318A | the mutant of isoform Arb43B displays a drastic decrease in enzymatic activity | Bacillus subtilis |
H318Q | enzymatic activity is completely lost in this mutant | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EGTA | isoform Arb43B shows a drastic decrease in the activity of the enzyme in the presence of 1 mm EGTA | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.076 | - |
linear 1,5-alpha-L-arabinan | mutant H318A of isoform Arb43B, pH and temperature not specified in the publication | Bacillus subtilis | |
0.111 | - |
linear 1,5-alpha-L-arabinan | wild type isoform Arb43B, pH and temperature not specified in the publication | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | isoform Arb43B possesses a cluster containing a calcium ion in the catalytic domain | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P42293 | - |
- |
Purification (Comment) | Organism |
---|---|
His-Trap column chromatography | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
linear 1,5-alpha-L-arabinan + H2O | - |
Bacillus subtilis | L-arabinose + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ABN | - |
Bacillus subtilis |
Arb43A | isoform, formally named AbnA | Bacillus subtilis |
Arb43B | isoform, formally named Abn2 | Bacillus subtilis |
endo-1,5-alpha-L-arabinanase | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.1 | - |
linear 1,5-alpha-L-arabinan | mutant H318A of isoform Arb43B, pH and temperature not specified in the publication | Bacillus subtilis | |
191.6 | - |
linear 1,5-alpha-L-arabinan | wild type isoform Arb43B, pH and temperature not specified in the publication | Bacillus subtilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00004 | - |
linear 1,5-alpha-L-arabinan | mutant H318A of isoform Arb43B, pH and temperature not specified in the publication | Bacillus subtilis | |
0.00172 | - |
linear 1,5-alpha-L-arabinan | wild type isoform Arb43B, pH and temperature not specified in the publication | Bacillus subtilis |