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Literature summary for 3.2.1.99 extracted from
- Biochemical characterization and gene expression of two endo-arabinanases from Penicillium chrysogenum 31B (2012), Appl. Microbiol. Biotechnol., 93, 1087-1096.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Penicillium chrysogenum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
gel filtration | Penicillium chrysogenum |
| 31000 | - |
1 * 31000, SDS-PAGE | Penicillium chrysogenum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium chrysogenum | - |
- |
- |
| Penicillium chrysogenum | Q5H7M8 | - |
- |
| Penicillium chrysogenum 31B | - |
- |
- |
| Penicillium chrysogenum 31B | Q5H7M8 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | no glycoprotein | Penicillium chrysogenum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| CM-Toyopearl 650 M column chromatography, Ni Sepharose 6 column chromatography, butyl-Toyopearl column chromatography, and Superdex 75 gel filtration | Penicillium chrysogenum |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 31000, SDS-PAGE | Penicillium chrysogenum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Abnc | - |
Penicillium chrysogenum |
| AbnS1 | - |
Penicillium chrysogenum |
| cold-adapted endo-arabinanase | - |
Penicillium chrysogenum |
| endo-arabinase | - |
Penicillium chrysogenum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Penicillium chrysogenum |
| 60 | - |
- |
Penicillium chrysogenum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | the enzyme maintains 100% of the initial activity by treatment it at 50°C for 1 h, while approximately 70% of the activity is lost at 60°C | Penicillium chrysogenum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | - |
Penicillium chrysogenum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | arabinanase activity drops to about 55% and 70% of the highest activity at pH 5.0 and 8.0, respectively | Penicillium chrysogenum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | - |
Penicillium chrysogenum |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Penicillium chrysogenum | expression of the gene encoding cold-adapted endo-arabinase Abnc is strongly induced by arabinose, arabinitol, and arabinan | up |
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