Application | Comment | Organism |
---|---|---|
industry | endo-1,5-alpha-L-arabinanases belonging to glycoside hydrolase family 43 are of great industrial interest for use in food technology, organic synthesis and biofuel production owing to their ability to catalyze the hydrolysis of alpha-1,5-arabinofuranosidic bonds in arabinose-containing polysaccharides | Thermotoga petrophila |
Cloned (Comment) | Organism |
---|---|
overexpression of His-tagged enzyme in Escherichia coli | Thermotoga petrophila |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, 0.0005 ml of protein solution containing 30 mg/ml in 25 mM Tris-HCl buffer, pH 7, 5, is mixed with 0.0005 ml of reservoir solution containing 0.1 M MES buffer, pH 6.5, 0.8 M ammonium sulfate, 0.1 M EDTA, 0.1 M L-proline and 5% v/v dioxane, X-ray diffraction structure determination and analysis at 2.86 A resolution | Thermotoga petrophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Thermotoga petrophila | the enzyme shows the ability to catalyze the hydrolysis of alpha-1,5-arabinofuranosidic bonds in arabinose-containing polysaccharides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga petrophila | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli | Thermotoga petrophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme shows the ability to catalyze the hydrolysis of alpha-1,5-arabinofuranosidic bonds in arabinose-containing polysaccharides | Thermotoga petrophila | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endo-1,5-alpha-L-arabinanase | - |
Thermotoga petrophila |
More | the enzyme belongs to the glycoside hydrolase family 43, GH43 | Thermotoga petrophila |