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Literature summary for 3.2.1.99 extracted from

  • Inacio, J.M.; de Sa-Nogueira, I.
    Characterization of abn2 (yxiA), encoding a Bacillus subtilis GH43 arabinanase, Abn2, and its role in arabino-polysaccharide degradation (2008), J. Bacteriol., 190, 4272-4280.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information expression of abn2 is stimulated by arabinan and pectin, arabinose is not the natural inducer. EDTA does not affect the activity Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
Abn2 overexpressed in Escherichia coli BL21(DE3) pLysS harboring pZI39 Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
additional information mutant strains IQB413 (DELTAabnA), IQB485 (DELTAabn2), and IQB486 (DELTAabnA DELTAabn2). Almost complete loss of activity in the DELTAabn2 DELTAabnA double mutant Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
additional information expression of abn2 is repressed by glucose. Glucose causes a 17- and 44fold repression of the abn2'-lacZ fusion expression during the exponential growth phase and the early postexponential phase, respectively Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52380
-
sequence analysis Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P42293
-
-
Bacillus subtilis 168T+ P42293
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration, more than 95% pure Bacillus subtilis

Storage Stability

Storage Stability Organism
-80°C, 20 mM Na-phosphate buffer, pH 7.4, 50 mM NaCl, 10% glycerol Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-alpha-L-arabinan + H2O Abn2, together with previously characterized AbnA, is responsible for the majority of the extracellular arabinan activity in Bacillus subtilis. Abn2 is monocistronic, and its expression is driven from two different promoters Bacillus subtilis ?
-
?
1,5-alpha-L-arabinan + H2O Abn2, together with previously characterized AbnA, is responsible for the majority of the extracellular arabinan activity in Bacillus subtilis. Abn2 is monocistronic, and its expression is driven from two different promoters Bacillus subtilis 168T+ ?
-
?
apple pectin + H2O
-
Bacillus subtilis ?
-
?
apple pectin + H2O
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Bacillus subtilis 168T+ ?
-
?
additional information shows no activity toward larchwood arabinogalactan, wheat arabinoxylan, and p-nitrophenyl-alpha-L-arabinofuranoside Bacillus subtilis ?
-
?
additional information shows no activity toward larchwood arabinogalactan, wheat arabinoxylan, and p-nitrophenyl-alpha-L-arabinofuranoside Bacillus subtilis 168T+ ?
-
?
red debranched arabinan + H2O
-
Bacillus subtilis ?
-
?
red debranched arabinan + H2O
-
Bacillus subtilis 168T+ ?
-
?
sugar beet arabinan + H2O
-
Bacillus subtilis ?
-
?
sugar beet arabinan + H2O
-
Bacillus subtilis 168T+ ?
-
?

Synonyms

Synonyms Comment Organism
Abn2
-
Bacillus subtilis
endo-alpha-1,5-L-arabinanase
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Bacillus subtilis
GH43 arabinanase
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Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
remains fully active after 30 min of preincubation at 50°C. After preincubation at 60°C, the residual activity is only 15% Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Bacillus subtilis

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus subtilis
-
-
7.37