Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | expression of abn2 is stimulated by arabinan and pectin, arabinose is not the natural inducer. EDTA does not affect the activity | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
Abn2 overexpressed in Escherichia coli BL21(DE3) pLysS harboring pZI39 | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant strains IQB413 (DELTAabnA), IQB485 (DELTAabn2), and IQB486 (DELTAabnA DELTAabn2). Almost complete loss of activity in the DELTAabn2 DELTAabnA double mutant | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | expression of abn2 is repressed by glucose. Glucose causes a 17- and 44fold repression of the abn2'-lacZ fusion expression during the exponential growth phase and the early postexponential phase, respectively | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52380 | - |
sequence analysis | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P42293 | - |
- |
Bacillus subtilis 168T+ | P42293 | - |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration, more than 95% pure | Bacillus subtilis |
Storage Stability | Organism |
---|---|
-80°C, 20 mM Na-phosphate buffer, pH 7.4, 50 mM NaCl, 10% glycerol | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,5-alpha-L-arabinan + H2O | Abn2, together with previously characterized AbnA, is responsible for the majority of the extracellular arabinan activity in Bacillus subtilis. Abn2 is monocistronic, and its expression is driven from two different promoters | Bacillus subtilis | ? | - |
? | |
1,5-alpha-L-arabinan + H2O | Abn2, together with previously characterized AbnA, is responsible for the majority of the extracellular arabinan activity in Bacillus subtilis. Abn2 is monocistronic, and its expression is driven from two different promoters | Bacillus subtilis 168T+ | ? | - |
? | |
apple pectin + H2O | - |
Bacillus subtilis | ? | - |
? | |
apple pectin + H2O | - |
Bacillus subtilis 168T+ | ? | - |
? | |
additional information | shows no activity toward larchwood arabinogalactan, wheat arabinoxylan, and p-nitrophenyl-alpha-L-arabinofuranoside | Bacillus subtilis | ? | - |
? | |
additional information | shows no activity toward larchwood arabinogalactan, wheat arabinoxylan, and p-nitrophenyl-alpha-L-arabinofuranoside | Bacillus subtilis 168T+ | ? | - |
? | |
red debranched arabinan + H2O | - |
Bacillus subtilis | ? | - |
? | |
red debranched arabinan + H2O | - |
Bacillus subtilis 168T+ | ? | - |
? | |
sugar beet arabinan + H2O | - |
Bacillus subtilis | ? | - |
? | |
sugar beet arabinan + H2O | - |
Bacillus subtilis 168T+ | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Abn2 | - |
Bacillus subtilis |
endo-alpha-1,5-L-arabinanase | - |
Bacillus subtilis |
GH43 arabinanase | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
remains fully active after 30 min of preincubation at 50°C. After preincubation at 60°C, the residual activity is only 15% | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Bacillus subtilis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus subtilis | - |
- |
7.37 |