Literature summary for 3.2.1.99 extracted from

Yamaguchi, A.; Tada, T.; Nakaniwa, T.; Kitatani, T.; Makoto, T.; Sakai, T.; Nishimura, K.
Crystallization and preliminary x-ray diffraction analysis of a thermostable endo-1,5-alpha-L-arabinanase from Bacillus thermodenitrificans TS-3 (2004), Acta Crystallogr. Sect. D, 60, 1149-1151.

Search for more literature for 3.2.1.99

Application

Application	Comment	Organism
nutrition	production of L-arabinose from plant arabinan at high temperatures	Geobacillus thermodenitrificans

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Bacillus subtilis strain MI112	Geobacillus thermodenitrificans

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapour-diffusion	Geobacillus thermodenitrificans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35000	-	SDS-PAGE	Geobacillus thermodenitrificans

Organism

Organism	UniProt	Comment	Textmining
Geobacillus thermodenitrificans	-	-	-
Geobacillus thermodenitrificans TS-3	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
hydrophobic chromatography, anion-exchange chromatography, gel filtration chromatography	Geobacillus thermodenitrificans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1,5-arabinan + H2O	-	Geobacillus thermodenitrificans	L-arabinose + ?	-	?
1,5-arabinan + H2O	-	Geobacillus thermodenitrificans TS-3	L-arabinose + ?	-	?

Synonyms

Synonyms	Comment	Organism
ABN	-	Geobacillus thermodenitrificans
endo-1,5-alpha-L-arabinanase	-	Geobacillus thermodenitrificans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
70	-	-	Geobacillus thermodenitrificans

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
75	-	4 h half-life	Geobacillus thermodenitrificans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Geobacillus thermodenitrificans

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Release 2023.1 (January 2023)