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Literature summary for 3.2.1.99 extracted from

  • Dunkel, M.P.H.; Amado, R.
    Purification and physico-chemical properties of an endo-1,5-alpha-L-arabinanase (EC 3.2.1.99) isolated from an Aspergillus niger pectinase preparation (1994), Carbohydr. Polym., 24, 247-263.
No PubMed abstract available
Search for more literature for 3.2.1.99

Application

Application Comment Organism
analysis
-
 Aspergillus niger
nutrition
-
 Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ completely Aspergillus niger
HgCl2 completely Aspergillus niger
iodoacetamide very slight inhibition Aspergillus niger
MgSO4 very slight inhibition Aspergillus niger
additional information no inhibition by CaCl2, EDTA, L-arabono-gamma-lactone, L-arabinose, D-arabitol Aspergillus niger
Zn2+
-
 Aspergillus niger
ZnSO4
-
 Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic data Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33900
-
 MALDI-TOF-MS Aspergillus niger
41000
-
 gel filtration Aspergillus niger
42500
-
 SDS-PAGE Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Aspergillus niger

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14
-
 substrate: branched sugar beet arabinan Aspergillus niger
81.1
-
 substrate: debranched sugar beet arabinan Aspergillus niger
90.2
-
 best substrate: linear 1,5-alpha-L-arabinan Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-alpha-L-arabinan + H2O best substrate, endo mechanism Aspergillus niger alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
 ?
1,5-alpha-L-arabinan + H2O best substrate: linear 1,5-alpha-L-haze arabinan Aspergillus niger alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
 ?
galactan + H2O very poor substrate Aspergillus niger ?
-
 ?
L-arabinan + H2O debranched and branched sugar beet arabinan Aspergillus niger L-arabino-oligosaccharides
-
 ?
additional information substrate specificity, overview Aspergillus niger ?
-
 ?

Synonyms

Synonyms Comment Organism
endo-ABA
-
 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 long incubation times, 26.5 h, at 40°C: 90% activity retained Aspergillus niger
45
-
 up to 45°C: more than 95% activity retained for 3 h Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5
-
 Aspergillus niger
4.8
-
 maximum Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 8 85% activity retained between, 67 h at 25°C Aspergillus niger