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Literature summary for 3.2.1.99 extracted from

  • Yoshihara, O.; Kaji, A.
    An endo-1,5-alpha-L-arabinase, which can disintegrate potato tissue (1983), Agric. Biol. Chem., 47, 1935-1940.
No PubMed abstract available
Search for more literature for 3.2.1.99

Inhibitors

Inhibitors Comment Organism Structure
Fe2+ IFO 3022 Bacillus subtilis
Fe3+ IFO 3022 Bacillus subtilis
Hg2+ IFO 3022, completely Bacillus subtilis
additional information IFO 3022: no inhibition by L-arabonic-gamma-lactone, D- and L-galactonic-gamma-lactones Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 IFO 3022, SDS-PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bacillus subtilis plays a role in degradation of plant tissues, macerating enzyme ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
 IFO 3022
-

Purification (Commentary)

Purification (Comment) Organism
IFO 3022 Bacillus subtilis

Source Tissue

Source Tissue Comment Organism Textmining
culture medium IFO 3022 Bacillus subtilis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.78
-
 IFO 3022 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-alpha-L-arabinan + H2O IFO 3022, best substrate Bacillus subtilis L-arabinose + alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara IFO 3022 ?
L-arabinan + H2O beet arabinan Bacillus subtilis L-arabino-oligosaccharides
-
 ?
additional information IFO 3022: potato disc macerating activity, able to loosen potato tissue Bacillus subtilis ?
-
 ?
additional information IFO 3022: inactive on p-nitrophenyl alpha-L-arabinofuranoside, p-nitrophenyl alpha-D-galactopyranoside, p-nitrophenyl beta-D-galactopyranoside, arabinogalactan, carboxymethyl cellulose, filter paper, pectin and polygalacturonic acid Bacillus subtilis ?
-
 ?
additional information plays a role in degradation of plant tissues, macerating enzyme Bacillus subtilis ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 IFO 3022 Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
3 9 IFO 3022 Bacillus subtilis