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Literature summary for 3.2.1.99 extracted from

  • Kaji, A.; Saheki, T.
    endo-Arabinanase from Bacillus subtilis F-11 (1975), Biochim. Biophys. Acta, 410, 354-360.
    View publication on PubMed
Search for more literature for 3.2.1.99

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ F-11 Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,5-alpha-L-arabinan + H2O Bacillus subtilis
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
 F-11
-
Bacillus subtilis F-11
-
 F-11
-

Purification (Commentary)

Purification (Comment) Organism
F-11 Bacillus subtilis

Source Tissue

Source Tissue Comment Organism Textmining
culture medium F-11 Bacillus subtilis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
48.8
-
 F-11 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-alpha-L-arabinan + H2O F-11 best substrate Bacillus subtilis alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose end-product ?
1,5-alpha-L-arabinan + H2O F-11 best substrate Bacillus subtilis alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose F-11: releases arabinose, arabinobiose, arabinotriose and higher oligosaccharides during hydrolysis, end-product are arabinose and arabinobiose ?
1,5-alpha-L-arabinan + H2O F-11 best substrate Bacillus subtilis F-11 alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose end-product ?
1,5-alpha-L-arabinan + H2O F-11 best substrate Bacillus subtilis F-11 alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose F-11: releases arabinose, arabinobiose, arabinotriose and higher oligosaccharides during hydrolysis, end-product are arabinose and arabinobiose ?
1,5-alpha-L-arabinan + H2O F-11 best substrate Bacillus subtilis L-arabinose + alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
 ?
1,5-alpha-L-arabinan + H2O F-11 best substrate Bacillus subtilis F-11 L-arabinose + alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
 ?
1,5-alpha-L-arabinan + H2O
-
 Bacillus subtilis ?
-
 ?
L-arabinan + H2O beet arabinan Bacillus subtilis L-arabino-oligosaccharides
-
 ?
L-arabinan + H2O random hydrolysis of L-arabinan Bacillus subtilis L-arabino-oligosaccharides
-
 ?
L-arabinan + H2O beet arabinan Bacillus subtilis F-11 L-arabino-oligosaccharides
-
 ?
additional information F-11: inactive on phenyl alpha-L-arabinofuranoside, p-nitrophenyl alpha-D-galactopyranoside, p-nitrophenyl beta-D-galactopyranoside, arabinoxylan, arabinogalactan and gum arabic Bacillus subtilis ?
-
 ?
additional information F-11: inactive on phenyl alpha-L-arabinofuranoside, p-nitrophenyl alpha-D-galactopyranoside, p-nitrophenyl beta-D-galactopyranoside, arabinoxylan, arabinogalactan and gum arabic Bacillus subtilis F-11 ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 F-11 Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
4 9 F-11 Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 10 F-11, stable between Bacillus subtilis
9
-
 F-11, most stable at Bacillus subtilis